Product namePAK peptide substrate
See all PAK1 proteins and peptides
Amino Acid Sequence
Additional sequence informationThe 10 amino acids of PAK peptide RRRLSFAEPG contain a serine/threonine protein kinase phosphorylation site in a common seven-residue epitope.
Our Abpromise guarantee covers the use of ab204872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
ReconstitutionDilute peptide in 20mM Tris-HCl, pH 7.5 solution to a final concentration of 1 mg/ml. For optimal storage, aliquot diluted product into smaller quantities and store at recommended temperature.
- Alpha PAK
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
- Information by UniProt
ab204872 has not yet been referenced specifically in any publications.