Product nameAnti-PAK1 antibody
See all PAK1 primary antibodies
DescriptionRabbit polyclonal to PAK1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Methanol-fixed HeLa cells; 293 and 3T3 cell extracts; Human breast carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/10 - 1/200.|
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
- Information by UniProt
- ADRB2 antibody
- Alpha PAK antibody
- Alpha-PAK antibody
All lanes : Anti-PAK1 antibody (ab131522) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 3T3 cell extract
Predicted band size: 61 kDa
Immunofluorescent analysis of methanol-fixed HeLa cells labelling PAK1 with ab131522 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling PAK1 with ab131522 at 1/50 dilution. Right panel was preincubated with blocking peptide.
This product has been referenced in:
- Wu XM et al. microRNA-542-5p protects against acute lung injury in mice with severe acute pancreatitis by suppressing the mitogen-activated protein kinase signaling pathway through the negative regulation of P21-activated kinase 1. J Cell Biochem 120:290-304 (2019). Read more (PubMed: 30216510) »
- Wen X et al. ROCK1 and LIM Kinase Modulate Retrovirus Particle Release and Cell-Cell Transmission Events. J Virol 88:6906-21 (2014). Read more (PubMed: 24696479) »