Anti-PAK1 antibody (ab137810)
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- References
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Overview
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Product name
Anti-PAK1 antibody
See all PAK1 primary antibodies -
Description
Rabbit polyclonal to PAK1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 245-520 of Human PAK1
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Positive control
- 293T, A431, H1299, HepG2, MOLT4 and Raji whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
Target
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Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1. -
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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Database links
- Entrez Gene: 533729 Cow
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 29431 Rat
- Omim: 602590 Human
- SwissProt: Q08E52 Cow
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
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Alternative names
- ADRB2 antibody
- Alpha PAK antibody
- Alpha-PAK antibody
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Images
Protocols
References
ab137810 has not yet been referenced specifically in any publications.