Anti-PAK1 antibody (ab137810)
Key features and details
- Rabbit polyclonal to PAK1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 antibody
See all PAK1 primary antibodies -
Description
Rabbit polyclonal to PAK1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 245-520 of Human PAK1
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Positive control
- 293T, A431, H1299, HepG2, MOLT4 and Raji whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
Target
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Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1. -
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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Database links
- Entrez Gene: 533729 Cow
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 29431 Rat
- Omim: 602590 Human
- SwissProt: Q08E52 Cow
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
see all -
Alternative names
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
Images
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Western blot - Anti-PAK1 antibody (ab137810)Anti-PAK1 antibody (ab137810) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS PAGE
References (0)
ab137810 has not yet been referenced specifically in any publications.
