Key features and details
- Rabbit polyclonal to PAK1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PAK1 antibody
See all PAK1 primary antibodies
DescriptionRabbit polyclonal to PAK1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Synthetic peptide within Human PAK1 aa 1-50. The exact sequence is proprietary. NP_002567.3
Database link: Q13153
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245414 was affinity purified using an epitope specific to PAK1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 61 kDa.|
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
- Information by UniProt
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
All lanes : Anti-PAK1 antibody (ab245414) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 10 seconds
PAK1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245414 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245414 at 0.1 µg/ml.
Lane 1: ab245414 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245414 has not yet been referenced specifically in any publications.