Anti-PAK1 + PAK2 + PAK3 antibody (ab131547)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 + PAK2 + PAK3 antibody
See all PAK1 + PAK2 + PAK3 primary antibodies -
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 421-425/400-404/419-423 (
RSTMV
) of Human PAK1 + PAK2 + PAK3 (UniProt Q13153/Q13177/O75914-2). -
Positive control
- HeLa cells; Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/200.
|
Notes |
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IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Relevance
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. -
Cellular localization
Cytoplasm. Recruited to focal adhesions upon activation -
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Omim: 300142 Human
- Omim: 602590 Human
- Omim: 605022 Human
- SwissProt: O75914 Human
- SwissProt: Q13153 Human
see all -
Alternative names
- Alpha PAK antibody
- Beta PAK antibody
- bPAK antibody
see all
Images
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling PAK1 + PAK2 + PAK3 with ab131547 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling PAK1 + PAK2 + PAK3 with ab131547 at 1/50 dilution (left) or ab131547 preincubated with blocking peptide (right).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131547 has not yet been referenced specifically in any publications.