Overview

  • Product name

    Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody
    See all PAK1 + PAK2 + PAK3 primary antibodies
  • Description

    Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T402)
  • Host species

    Rabbit
  • Specificity

    ab30577 recognises PAK1 + PAK2 + PAK3 (phospho T402).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic Phosphopeptide corresponding to amino acid residues surrounding the phospho Thr402 of rat p21 Activated Kinase 2 (PAK2). The peptide sequence used is identical in PAK1, 2 and 3.

  • Positive control

    • Rat hippocampal lysate
  • General notes

    Note: Thr402 in PAK2 corresponds to Thr423 in human PAK1.


    Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.

Properties

Applications

Our Abpromise guarantee covers the use of ab30577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).

Target

  • Relevance

    In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
  • Cellular localization

    Cytoplasm. Recruited to focal adhesions upon activation
  • Database links

  • Alternative names

    • Alpha PAK antibody
    • Beta PAK antibody
    • bPAK antibody
    • CDKN1A antibody
    • Gamma PAK antibody
    • hPAK3 antibody
    • MRX30 antibody
    • MRX47 antibody
    • Oligophrenin 3 antibody
    • OPHN3 antibody
    • P21 (CDKN1A) activated kinase 2 antibody
    • P21 (CDKN1A) activated kinase 3 antibody
    • p21 activated kinase 1 antibody
    • p21 activated kinase 2 antibody
    • p21 activated kinase 3 antibody
    • P21 protein (Cdc42/Rac) activated kinase 1 antibody
    • P21 protein (Cdc42/Rac) activated kinase 2 antibody
    • P21 protein (Cdc42/Rac) activated kinase 3 antibody
    • P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast) antibody
    • P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • P58 antibody
    • P65 PAK antibody
    • p65-PAK antibody
    • PAK 2 antibody
    • PAK 3 antibody
    • PAK1 antibody
    • PAK2 antibody
    • PAK3 antibody
    • PAK3beta antibody
    • PAK65 antibody
    • PAKalpha antibody
    • PAKgamma antibody
    • S6/H4 kinase antibody
    • Serine/threonine protein kinase PAK 1 antibody
    • Serine/threonine protein kinase PAK 2 antibody
    • Serine/threonine protein kinase PAK 3 antibody
    • Serine/threonine protein kinase PAK1 antibody
    • Serine/threonine protein kinase PAK2 antibody
    • Serine/threonine protein kinase PAK3 antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog, yeast antibody
    see all

Images

  • All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilution

    Lane 1 : untreated rat hippocampal lysate
    Lane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control)

    Predicted band size: 60 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

References

ab30577 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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