Key features and details
- Rabbit polyclonal to PAK1 (phospho S199)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAK1 (phospho S199) antibody
See all PAK1 primary antibodies
DescriptionRabbit polyclonal to PAK1 (phospho S199)
Specificityab192814 detects endogenous levels of PAK1 only when phosphorylated at serine 199.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human PAK1 (phospho S199) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of Serine 199 (T-K-S(p)-V-I).
Database link: Q13153
- Lysate of starved LOVO cells and Human placenta tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
- Information by UniProt
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
All lanes : Anti-PAK1 (phospho S199) antibody (ab192814) at 1/500 dilution
Lane 1 : Lysate of starved LOVO cells
Lane 2 : Lysate of starved LOVO cells with antigen-specific peptide
Predicted band size: 61 kDa
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAK1 (phospho S199) using ab192814 at a 1/50 dilution, the right panel was treated with blocking peptide.
ab192814 has been referenced in 1 publication.
- Feng Y et al. Alpha-1 Antitrypsin Prevents the Development of Preeclampsia Through Suppression of Oxidative Stress. Front Physiol 7:176 (2016). PubMed: 27303303