Overview

  • Product name
    Anti-PAK1 (phospho S20) antibody [EP655Y]
    See all PAK1 primary antibodies
  • Description
    Rabbit monoclonal [EP655Y] to PAK1 (phospho S20)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PAK1 (phospho S20). Numbering corresponds to mature PAK1 without Initiator methionine.

  • Positive control
    • HeLa cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    IP: 1/50.
    WB: 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

    Is unsuitable for Flow Cyt or IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated when activated by CDC42/p21 and RAC1.
    • Cellular localization
      Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADRB2 antibody
      • Alpha PAK antibody
      • Alpha-PAK antibody
      • MGC130000 antibody
      • MGC130001 antibody
      • p21 activated kinase 1 antibody
      • p21 protein (Cdc42/Rac) activated kinase 1 antibody
      • p21-activated kinase 1 antibody
      • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
      • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
      • p65 PAK antibody
      • p65-PAK antibody
      • P68-PAK antibody
      • PAK alpha antibody
      • PAK-1 antibody
      • Pak1 antibody
      • PAK1_HUMAN antibody
      • Paka antibody
      • PAKalpha antibody
      • Protein kinase MUK2 antibody
      • Rac/p21-activated kinase antibody
      • Serine/threonine-protein kinase PAK 1 antibody
      • STE20 homolog yeast antibody
      see all

    Images

    • All lanes : Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates with 5% NFDM/TBST
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes Whole cell lysates with 5% NFDM/TBST
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes. Then the membrane was incubated with phosphatase. with 5% NFDM/TBST

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 61 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      We are unable to define the extra bands

    References

    ab51244 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab51244.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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