Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP655Y] to PAK1 (phospho S20) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free
See all PAK1 primary antibodies
DescriptionRabbit monoclonal [EP655Y] to PAK1 (phospho S20) - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human PAK1.
- WB: HeLa cell lysate.
ab235117 is the carrier-free version of ab51244 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab235117 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab235117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).|
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
- Information by UniProt
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
All lanes : Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) wole cell lysates with 5% NFDM/TBST
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes whole cell lysates with 5% NFDM/TBST
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes. Then the membrane was incubated with phosphatase with 5% NFDM/TBST
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51244).
We are unable to define the extra bands.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235117 has not yet been referenced specifically in any publications.