Recombinant
RabMAb

Recombinant Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free (ab235117)

Overview

  • Product name

    Anti-PAK1 (phospho S20) antibody [EP655Y] - BSA and Azide free
    See all PAK1 primary antibodies
  • Description

    Rabbit monoclonal [EP655Y] to PAK1 (phospho S20) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PAK1.

  • Positive control

    • WB: HeLa cell lysate.
  • General notes

    ab235117 is the carrier-free version of ab51244 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab235117 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab235117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated when activated by CDC42/p21 and RAC1.
    • Cellular localization

      Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADRB2 antibody
      • Alpha PAK antibody
      • Alpha-PAK antibody
      • MGC130000 antibody
      • MGC130001 antibody
      • p21 activated kinase 1 antibody
      • p21 protein (Cdc42/Rac) activated kinase 1 antibody
      • p21-activated kinase 1 antibody
      • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
      • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
      • p65 PAK antibody
      • p65-PAK antibody
      • P68-PAK antibody
      • PAK alpha antibody
      • PAK-1 antibody
      • Pak1 antibody
      • PAK1_HUMAN antibody
      • Paka antibody
      • PAKalpha antibody
      • Protein kinase MUK2 antibody
      • Rac/p21-activated kinase antibody
      • Serine/threonine-protein kinase PAK 1 antibody
      • STE20 homolog yeast antibody
      see all

    Images

    • All lanes : Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) wole cell lysates with 5% NFDM/TBST
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes whole cell lysates with 5% NFDM/TBST
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes. Then the membrane was incubated with phosphatase with 5% NFDM/TBST

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 61 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51244).

      We are unable to define the extra bands.

    References

    ab235117 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab235117.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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