Key features and details
- Rabbit polyclonal to PAK1 (phospho S204)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PAK1 (phospho S204) antibody
See all PAK1 primary antibodies
DescriptionRabbit polyclonal to PAK1 (phospho S204)
Specificityab79503 detects endogenous levels of PAK1 only when phosphorylated at serine 204.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of serine 204 (TRSPVI)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
- Information by UniProt
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
All lanes : Anti-PAK1 (phospho S204) antibody (ab79503) at 1/1000 dilution
Lane 1 : extracts from 3T3 cells (treated
with UV for 15 mins)
Lane 2 : extracts from 3T3 cells (treated
with UV for 15 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Immunohistochemistry analysis of paraffin-embedded human brain tissue, stained with ab79503, at 1/100 dilution, in the absence and presence of the immunising peptide
ab79503 has been referenced in 1 publication.
- Vaquié A et al. Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration. Cell Rep 27:3152-3166.e7 (2019). PubMed: 31189102