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    pak2-antibody-ab245542.pdf

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Cell Biology Apoptosis Intracellular Kinases
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Anti-PAK2 antibody (ab245542)

  • Datasheet
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Abpromise

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Western blot - Anti-PAK2 antibody (ab245542)
  • Immunoprecipitation - Anti-PAK2 antibody (ab245542)

Key features and details

  • Rabbit polyclonal to PAK2
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Protein
Product image
Recombinant Human PAK2 protein (ab125597)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PAK2 antibody
    See all PAK2 primary antibodies
  • Description

    Rabbit polyclonal to PAK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PAK2 aa 125-175. The exact sequence is proprietary. NP_002568.2
    Database link: Q13177

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IP: PAK2 IP in HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245542 was affinity purified using an epitope specific to PAK2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human PAK2 protein (ab125597)
  • Related Products

    • Recombinant Human PAK2 protein (ab125597)

Applications

Our Abpromise guarantee covers the use of ab245542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000.

Target

  • Function

    The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
  • Tissue specificity

    Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
    During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
    Ubiquitinated, leading to its proteasomal degradation.
    PAK-2p34 is myristoylated.
  • Cellular localization

    Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
  • Target information above from: UniProt accession Q13177 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5062 Human
    • GenBank: NP_002568.2 Human
    • Omim: 605022 Human
    • SwissProt: Q13177 Human
    • Unigene: 518530 Human
    • Alternative names

      • C-t-PAK2 antibody
      • CB422 antibody
      • EC 2.7.11.1 antibody
      • Gamma PAK antibody
      • Gamma-PAK antibody
      • hPAK65 antibody
      • Kinase antibody
      • p21 (CDKN1A) activated kinase 2 antibody
      • p21 (CDKN1A)-activated kinase 2a antibody
      • p21 activated kinase 2 antibody
      • p21 protein (Cdc42/Rac)-activated kinase 2 antibody
      • p21 protein Cdc42 Rac activated kinase 2 antibody
      • p21-activated kinase 2 antibody
      • p21-activated kinase, 65-KD antibody
      • p21-activated protein kinase I antibody
      • p21CDKN1A activated kinase 2 antibody
      • p27 antibody
      • p34 antibody
      • p58 antibody
      • p65PAK antibody
      • PAK 2 antibody
      • PAK-2 antibody
      • PAK-2p34 antibody
      • Pak2 antibody
      • PAK2_HUMAN antibody
      • PAK65 antibody
      • PAKgamma antibody
      • S6 H4 kinase antibody
      • S6/H4 kinase antibody
      • Serine threonine protein kinase PAK 2 antibody
      • Serine/threonine protein kinase PAK 2 antibody
      see all

    Images

    • Western blot - Anti-PAK2 antibody (ab245542)
      Western blot - Anti-PAK2 antibody (ab245542)
      All lanes : Anti-PAK2 antibody (ab245542) at 0.04 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-PAK2 antibody (ab245542)
      Immunoprecipitation - Anti-PAK2 antibody (ab245542)

      PAK2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

      ab245542 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.

      Lane 1: ab245542 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.
      Chemiluminescence detection: 10 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab245542? Please let us know so that we can cite the reference in this datasheet.

    ab245542 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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