Western blot abreview for Anti-PAK2 antibody [EP796Y]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
30 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1:1000 in 5% milk
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: CST - Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Negative control
n/a
Exposure
30 second(s)
Bands
Specific: 62 kDa
Positive control
our samples

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Submitted Feb 27 2019

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