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Cell Biology Apoptosis Intracellular Kinases
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RecombinantRabMAb

Recombinant Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)

  • Datasheet
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Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)
  • Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR658(2)] to PAK2 (phospho S20)
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant Human PAK2 protein (ab125597)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PAK2 (phospho S20) antibody [EPR658(2)]
    See all PAK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR658(2)] to PAK2 (phospho S20)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 20 of human PAK2

  • Positive control

    • HeLa cell lysate treated with calyculin A
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR658(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other

Associated products

  • Alternative Versions

    • Anti-PAK2 (phospho S20) antibody [EPR658(2)] - BSA and Azide free (ab247470)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human PAK2 protein (ab125597)

Applications

Our Abpromise guarantee covers the use of ab76419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
    • Tissue specificity

      Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
      During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
      Ubiquitinated, leading to its proteasomal degradation.
      PAK-2p34 is myristoylated.
    • Cellular localization

      Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
    • Target information above from: UniProt accession Q13177 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5062 Human
      • GenBank: NP_002568.2 Human
      • Omim: 605022 Human
      • SwissProt: Q13177 Human
      • Unigene: 518530 Human
      • Alternative names

        • C-t-PAK2 antibody
        • CB422 antibody
        • EC 2.7.11.1 antibody
        • Gamma PAK antibody
        • Gamma-PAK antibody
        • hPAK65 antibody
        • Kinase antibody
        • p21 (CDKN1A) activated kinase 2 antibody
        • p21 (CDKN1A)-activated kinase 2a antibody
        • p21 activated kinase 2 antibody
        • p21 protein (Cdc42/Rac)-activated kinase 2 antibody
        • p21 protein Cdc42 Rac activated kinase 2 antibody
        • p21-activated kinase 2 antibody
        • p21-activated kinase, 65-KD antibody
        • p21-activated protein kinase I antibody
        • p21CDKN1A activated kinase 2 antibody
        • p27 antibody
        • p34 antibody
        • p58 antibody
        • p65PAK antibody
        • PAK 2 antibody
        • PAK-2 antibody
        • PAK-2p34 antibody
        • Pak2 antibody
        • PAK2_HUMAN antibody
        • PAK65 antibody
        • PAKgamma antibody
        • S6 H4 kinase antibody
        • S6/H4 kinase antibody
        • Serine threonine protein kinase PAK 2 antibody
        • Serine/threonine protein kinase PAK 2 antibody
        see all

      Images

      • Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)
        Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)
        All lanes : Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419) at 1/20000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HeLa cell lysate treated with calyculin A

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

        Predicted band size: 58 kDa
        Observed band size: 58 kDa

      • Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)
        Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab76419? Please let us know so that we can cite the reference in this datasheet.

      ab76419 has been referenced in 1 publication.

      • Wang T  et al. Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Cell 168:890-903.e15 (2017). PubMed: 28162770

      Customer reviews and Q&As

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