Recombinant
Recombinant Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-PAK2 (phospho S20) antibody [EPR658(2)]
See all PAK2 primary antibodies -
Description
Rabbit monoclonal [EPR658(2)] to PAK2 (phospho S20) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Serine 20 of human PAK2
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Positive control
- HeLa cell lysate treated with calyculin A
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR658(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/20000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). | |
| ICC/IF | 1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. -
Tissue specificity
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsFull length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated. -
Cellular localization
Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. - Information by UniProt
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Database links
- Entrez Gene: 5062 Human
- GenBank: NP_002568.2 Human
- Omim: 605022 Human
- SwissProt: Q13177 Human
- Unigene: 518530 Human
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Alternative names
- C-t-PAK2 antibody
- CB422 antibody
- EC 2.7.11.1 antibody
see all
Images
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![Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)](https://www.abcam.com/ps/products/76/ab76419/Images/ab76419-91822-anti-pak2-phospho-s20-antibody-epr6582-western-blot.gif)
Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)All lanes : Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Datasheets and documents
References
This product has been referenced in:
- Wang T et al. Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Cell 168:890-903.e15 (2017). Read more (PubMed: 28162770) »
