Recombinant Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)
![Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)](https://www.abcam.com/ps/products/76/ab76419/Images/ab76419-91822-anti-pak2-phospho-s20-antibody-epr6582-western-blot.gif "Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR658(2)] to PAK2 (phospho S20)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PAK2 (phospho S20) antibody [EPR658(2)]
See all PAK2 primary antibodies -
Description
Rabbit monoclonal [EPR658(2)] to PAK2 (phospho S20) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Serine 20 of human PAK2
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Positive control
- HeLa cell lysate treated with calyculin A
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR658(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/20000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. -
Tissue specificity
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsFull length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated. -
Cellular localization
Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. - Information by UniProt
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Database links
- Entrez Gene: 5062 Human
- GenBank: NP_002568.2 Human
- Omim: 605022 Human
- SwissProt: Q13177 Human
- Unigene: 518530 Human
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Alternative names
- C-t-PAK2 antibody
- CB422 antibody
- EC 2.7.11.1 antibody
see all
Images
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Western blot - Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)All lanes : Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa -
![Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)](https://www.abcam.com/ps/products/76/ab76419/Images/ab76419-2-benefits-of-recombinant-antibodies.png)
Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)
Datasheets and documents
References (1)
ab76419 has been referenced in 1 publication.
- Wang T et al. Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Cell 168:890-903.e15 (2017). PubMed: 28162770
