Overview

  • Product name
  • Description
    Rabbit polyclonal to PAK4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Mouse PAK4 aa 186-206 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8BTW9

  • Positive control
    • MCF7 cell line lysates; Neuro-2a cells and cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab173315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
  • Tissue specificity
    Highest expression in prostate, testis and colon.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on serine residues when activated by CDC42/p21.
    Phosphorylated on tyrosine residues upon stimulation of FGFR2.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1142 antibody
    • p21 activated kinase 4 antibody
    • p21 Cdc42/Rac1-actiated kinase 4 antibody
    • P21 protein (Cdc42/Rac) activated kinase 4 antibody
    • p21(CDKN1A) activated kinase 4 antibody
    • p21-activated kinase 4 antibody
    • PAK 4 antibody
    • PAK-4 antibody
    • Pak4 antibody
    • PAK4_HUMAN antibody
    • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
    • Serine threonine kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK4 antibody
    • Serine/threonine-protein kinase PAK 4 antibody
    see all

Images

  • Anti-PAK4 antibody (ab173315) at 1/100 dilution + Neuro-2a cell line lysates at 35 µg

    Predicted band size: 65 kDa

  • Anti-PAK4 antibody (ab173315) at 1/100 dilution + MCF-7 cell line lysates at 35 µg

    Predicted band size: 65 kDa

  • Flow cytometric analysis of Neuro-2a cells with ab173315 at 1/10 dilution (right histogram), compared with a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab173315 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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