Product nameAnti-PAK4 antibody
See all PAK4 primary antibodies
DescriptionRabbit polyclonal to PAK4
SpecificityThis antibody recognises PAK4. The antibody does not cross react with other PAKs.
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to the N terminus of human PAK4.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa.|
|IP||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 20 µg/ml.|
FunctionActivates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
Tissue specificityHighest expression in prostate, testis and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on serine residues when activated by CDC42/p21.
Phosphorylated on tyrosine residues upon stimulation of FGFR2.
- Information by UniProt
- KIAA1142 antibody
- p21 activated kinase 4 antibody
- p21 Cdc42/Rac1-actiated kinase 4 antibody
Anti-PAK4 antibody (ab19007) at 1 µg/ml + COS cell lysate
Predicted band size: 64 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Bergeron V et al. The P21-activated kinase PAK4 is implicated in fatty-acid potentiation of insulin secretion downstream of free fatty acid receptor 1. Islets 8:157-164 (2016). Read more (PubMed: 27700527) »
- Fiedler J et al. MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124:720-30 (2011). WB, IHC-Fr ; Mouse . Read more (PubMed: 21788589) »