Product nameAnti-PAK5 antibody
See all PAK5 primary antibodies
DescriptionRabbit polyclonal to PAK5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide from the C terminal region of Human PAK5.
- Human brain tissue; Rat brain cells extracts
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab110069 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab110069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 81 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThe activated kinase acts on a variety of targets.
Tissue specificityPredominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21.
- Information by UniProt
- EC 184.108.40.206 antibody
- KIAA1264 antibody
- MGC26232 antibody
All lanes : Anti-PAK5 antibody (ab110069) at 1/500 dilution
Lane 1 : extracts from Rat brain cells
Lane 2 : extracts from Rat brain cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 81 kDa
Immunohistochemical analysis of paraffin embedded Human brain tissue, using a 1/50 dilution of ab110069 with (right) or without (left) the immunizing peptide.
This product has been referenced in:
- Han K et al. PAK5 overexpression is associated with lung metastasis in osteosarcoma. Oncol Lett 15:2202-2210 (2018). Read more (PubMed: 29434926) »
- Aburatani T et al. High expression of P21-activated kinase 5 protein is associated with poor survival in gastric cancer. Oncol Lett 14:404-410 (2017). Read more (PubMed: 28693183) »