Product nameAnti-PAK5 antibody
See all PAK5 primary antibodies
DescriptionRabbit polyclonal to PAK5
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: PAK5 transiently transfected Cos-7 cell lysate. ICC/IF: SKNSH cell line. IHC-P: Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab37753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|WB||1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).|
FunctionThe activated kinase acts on a variety of targets.
Tissue specificityPredominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21.
- Information by UniProt
- EC 184.108.40.206 antibody
- KIAA1264 antibody
- MGC26232 antibody
Immunohistochemistry (Formalin/PFa-fixed paraffin-embedded sections) analysis of human brain tissue labelling PAK5 with ab37753. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.
ab37753 stained SKNSH cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37753 at 1/50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-PAK5 antibody (ab37753) at 1/100 dilution
Lane 1 : COS7 cells (negative control)
Lane 2 : transiently transfected COS7 cells with PAK1
Lane 3 : transiently transfected COS7 cells with PAK2
Lane 4 : transiently transfected COS7 cells with PAK4
Lane 5 : transiently transfected COS7 cells with PAK5
Lane 6 : transiently transfected COS7 cells with PAK6
Predicted band size: 81 kDa
Observed band size: 81 kDa
Additional bands at: 37 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
ab37753 has not yet been referenced specifically in any publications.