Product nameAnti-PAK6 antibody
See all PAK6 primary antibodies
DescriptionRabbit polyclonal to PAK6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Chimpanzee, Macaque monkey
- WB: Transiently transfected Cos-7 cell lysate. ICC/IF: LoVo cells. IHC-P: Human Cerebral Cortex (normal) tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab37749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionThe activated kinase acts on a variety of targets.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21.
- Information by UniProt
- CDKN1A activated kinase 6 antibody
- EC 188.8.131.52 antibody
- p21 activated protein kinase 6 antibody
All lanes : Anti-PAK6 antibody (ab37749)
Lane 1 : Negative control
Lane 2 : PAK1-expressing cell lysate
Lane 3 : PAK2-expressing cell lysate
Lane 4 : PAK4-expressing cell lysate
Lane 5 : PAK5-expressing cell lysate
Lane 6 : PAK6-expressing cell lysate
Predicted band size: 75 kDa
Observed band size: 75 kDa
Lanes 2-6 contain lysates from transiently transfected COS7 cells.
IHC image of PAK6 staining in Human cerebral Cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37749, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab37749 stained LoVo cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37749 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.