Product nameAnti-PAK6 antibody
See all PAK6 primary antibodies
DescriptionRabbit polyclonal to PAK6
SpecificityThe antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein. This reactivity is specifically blocked by pre-incubation with PAK6 N-terminal peptide (immunogen) and is not affected by lambda phosphatase treatment.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.
General notesDo not aliquot.
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab41431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
FunctionThe activated kinase acts on a variety of targets.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21.
- Information by UniProt
- CDKN1A activated kinase 6 antibody
- EC 220.127.116.11 antibody
- p21 activated protein kinase 6 antibody
All lanes : Anti-PAK6 antibody (ab41431) at 1/1000 dilution
Lane 1 : Human PAK6 recombinant protein phosphorylated by ERK2
Lane 2 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 3 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide
Lane 4 : Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (ab41431 immunogen)
Predicted band size: 75 kDa
Observed band size: 75 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
ab41431 has not yet been referenced specifically in any publications.