Key features and details
- Rabbit polyclonal to PAK6
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAK6 antibody
See all PAK6 primary antibodies
DescriptionRabbit polyclonal to PAK6
Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide (Human) of 13 amino acids from near the centre of PAK6
- Raji cells
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
|ICC||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionThe activated kinase acts on a variety of targets.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21.
- Information by UniProt
- CDKN1A activated kinase 6 antibody
- EC 18.104.22.168 antibody
- p21 activated protein kinase 6 antibody
Lane 1 : Anti-PAK6 antibody (ab62511) at 1 µg/ml
Lane 2 : Anti-PAK6 antibody (ab62511) at 2 µg/ml
Lane 3 : Anti-PAK6 antibody (ab62511) at 4 µg/ml
All lanes : Raji lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Immunocytochemistry of Raji cells labelled with ab62511 at 10 µg/ml.
Immunofluorescence of PAK6 in Raji cells using ab62511 at 10 ug/ml.
ab62511 has been referenced in 1 publication.
- Bastea LI et al. Protein kinase D-mediated phosphorylation at Ser99 regulates localization of p21-activated kinase 4. Biochem J 455:251-60 (2013). PubMed: 23841590