Product nameAnti-PAK6 (phospho S165) antibody
See all PAK6 primary antibodies
DescriptionRabbit polyclonal to PAK6 (phospho S165)
SpecificityPhospho-Ser165-specific. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-Ser165-PAK6 peptide and also by treatment with lambda phosphatase.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phospho-Ser165-PAK6 peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.
General notesDo not aliquot.
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was cross-adsorbed to unphosphorylated-Ser165-PAK6 peptide, then affinity purified using phospho-Ser165-PAK6 peptide (immunogen).
Our Abpromise guarantee covers the use of ab41432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
FunctionThe activated kinase acts on a variety of targets.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21.
- Information by UniProt
- CDKN1A activated kinase 6 antibody
- EC 188.8.131.52 antibody
- p21 activated protein kinase 6 antibody
Lanes 1-4 : Anti-PAK6 antibody (ab41431) at 1/1000 dilution
Lanes 5-8 : Anti-PAK6 (phospho S165) antibody (ab41432) at 1/1000 dilution
Lanes 1 & 5 : Human PAK6 recombinant protein phosphorylated by ERK2
Lane 2 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 3 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide
Lane 4 : Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (ab41431 immunogen)
Lane 6 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 7 : Human PAK6 recombinant protein phosphorylated by ERK2 with phospho-S165-PAK6 peptide (ab41432 immunogen)
Lane 8 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific phospho-serine peptide
Predicted band size: 75 kDa
Observed band size: 75 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
ab41432 has not yet been referenced specifically in any publications.