Product nameAnti-Palladin antibody [EPR9460(B)]
See all Palladin primary antibodies
DescriptionRabbit monoclonal [EPR9460(B)] to Palladin
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues near the N terminus of Human Palladin (UniProt Q8WX93).
- HeLa, 293T, PC3 and Caco-2 cell lysates; HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 151 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceCytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci (from SwissProt).
Cellular localizationCytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
- CGI 151 antibody
- CGI151 antibody
- KIAA0992 antibody
All lanes : Anti-Palladin antibody [EPR9460(B)] (ab154827) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : PC3 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 151 kDa
Flow cytometric analysis of permeabilized HeLa cells labeling Palladin with ab154827 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
ab154827 has not yet been referenced specifically in any publications.