Overview

  • Product name
  • Description
    Goat polyclonal to Pallidin
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: EREKQLTARPAKRM, corresponding to C terminal amino acids 159-172 of Human Pallidin.

  • Positive control
    • A431 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab10145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution >1/32000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 23-24 kDa (predicted molecular weight: 20 kDa according to NP_036520).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. May play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process.
    • Tissue specificity
      Widely expressed.
    • Post-translational
      modifications
      Phosphorylated.
    • Cellular localization
      Cytoplasm. Endomembrane system. It can exist as a soluble protein as well as a peripheral membrane protein.
    • Information by UniProt
    • Database links
    • Alternative names
      • Bloc1s6 antibody
      • iogenesis of lysosome-related organelles complex 1 subunit 6 antibody
      • PA antibody
      • Pallid (mouse) homolog antibody
      • PALLID antibody
      • Pallid protein antibody
      • Pallid protein homolog antibody
      • Pallidin antibody
      • Pallidin homolog (mouse) antibody
      • PLDN antibody
      • PLDN_HUMAN antibody
      • Syntaxin 13 binding protein antibody
      • Syntaxin 13-interacting protein antibody
      see all

    Images

    • ab10145 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

      ab10145 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References

    ab10145 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question

    Back in October 2004, we received an order of Pallidin antibody (Catalog No. ab10145; Lot No. 81163) from your company. We tested the quality of this lot and found *one major* band at the expected position, approximately 23-25 Kda, and hardly any extra bands. We used 2ug/ml antibody in one filter - see attachment TIFF file entitled "PLDN-Test 1.tiff" (protein loading 5ug/lane; PLDN: 2ug/ml and 0.5ug/ml respectively). More recently we received the same Pallidin antibody but a different lot (Lot No. 82307) and tested the quality of Pallidin antibody (same procedure as before) and got very different results. We found that there are multiple *faint* bands at 23-25 Kda, the desired position. In addition, there are several other more intense bands at higher molecular weight positions. See attachment file: "PLDN-Test 2.tiff" (protein loading12ug/lane; PLDN 2ug/ml in one filter, total 36 human postmortem brain samples in 4 filters). We have used this second lot of antibody twice now, and we are quite sure of both our technique and that the two antibodies give very different banding patterns. We have expended considerable time, energy, money, and protein samples in the process. Since the quality of this second batch (Lot No. 82307) of Pallidin antibody is not acceptable, we request that you ship us without charge, by overnight courier, Pallidin antibody from Lot 81163, which has worked well. This would go a long way in lessening our disappointment with the lot to lot variability at ABCAM. We did not think it necessary, but in future when ordering from ABCAM, we will specify the same lot number when reordering. As we are competing with multiple other research groups in characterizing this macromolecular complex involved in schizophrenia, we would appreciate the replacement as soon as possible.

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    Answer

    Thank you for your e-mail. We are very sorry to hear that you are having problem with this antibody. We have checked our order records and stock details, and found that the two batches (81163 and 82307) are actually from exactly the same bleed and purification. The batch numbers (for each of our products) are generated by our computer system and correspond with the date when we put the vials into our stock. We have another batch number for internal use only which clearly indicates the bleed numbers. We still have plenty of this bleed and there is no other. It may well be that the antibody you received second time may have gone off during shipping and /or storage. We could send you a free of charge aliquot, there is no problem. Would you be so kind to confirm your Abcam Order number or Purchase Order Number. We want to make sure that we will send the replacement vial to the right place. Your institute is very big and the address you provided does not completely match with that one on the order form for this particular antibody. We look forward to hearing from you soon.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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