Product nameAnti-Pallidin antibody
See all Pallidin primary antibodies
DescriptionRabbit polyclonal to Pallidin
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- WB: A431 and HEK-293T whole cell lysates. IHC-P: Human pancreatic cancer tissue. IP: HEK-293 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 20, 9 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/2000.|
FunctionInvolved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. May play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process.
Tissue specificityWidely expressed.
Cellular localizationCytoplasm. Endomembrane system. It can exist as a soluble protein as well as a peripheral membrane protein.
- Information by UniProt
- Bloc1s6 antibody
- iogenesis of lysosome-related organelles complex 1 subunit 6 antibody
- PA antibody
All lanes : Anti-Pallidin antibody (ab230361) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 20, 9 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Paraffin-embedded human pancreatic cancer tissue stained for Pallidin using ab230361 at 1/100 dilution in immunohistochemical analysis.
Pallidin was immunoprecipitated from 500 µg HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with 4 µg ab230361.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in HEK-293 whole cell lysate.
Lane 2: ab230361 IP in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody at 1/50000 dilution.
ab230361 has not yet been referenced specifically in any publications.