Product namePalmitoylated Protein Assay Kit (Green)
See all Palmitoylated Protein kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (qualitative)
Palmitoylated Protein Activity Assay Kit (Green) (ab273282) is a highly specific, simple and robust method for labeling and detection of palmitoylated proteins. The kit uses a modified Palmitic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This post translational modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Palmitoylated proteins or detection by FACS for quantitative studies.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applicationsSuitable for: Flow Cyt, FMmore details
PlatformFlow cytometer, Fluorescence microscope
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Copper Reagent (100X) 1 x 100µl Fixative Solution 1 x 10ml Fluorescent Azide (100X) 1 x 100µl Palmitic Acid Label (1000X) 1 x 10µl Permeabilization Buffer (10X) 1 x 25ml Reducing Agent (20X) 1 x 500µl Total DNA Stain (1000X) 1 x 20µl Wash Buffer (10X) 1 x 25ml
Our Abpromise guarantee covers the use of ab273282 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.|
|FM||Use at an assay dependent concentration.|
Metabolic labeling of palmitic acid on proliferating Jurkat cells. 1 x 106 cells incubated overnight with fresh aliquots of media containing Palmitic Acid Label. Analysis of Negative Control (Black), Background (Fluorescent Azide only, Green), Positive Control (Palmitic Acid Label and Fluorescent Azide, Pink). Signal measured in FL-1 channel clearly shows the Palmitic Acid Labeling of protein.
Metabolic labeling of palmitic acid on proliferating HeLa cells. 1 x 105 cells were incubated overnight with fresh aliquots of media containing Palmitic Acid Label. Upper panel corresponds to the Azide only Background fluorescence. The lower panel shows green fluorescence corresponds to the Palmitic Acid Label labeling. Nuclear staining in both panels confirms that green signal is a result of Palmitic Acid Label incorporation.
ab273282 has not yet been referenced specifically in any publications.