Key features and details
- Rabbit polyclonal to PAMCI
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PAMCI antibody
See all PAMCI primary antibodies
DescriptionRabbit polyclonal to PAMCI
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human breast tissue, RT4 cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.|
FunctionMay play a role in regulating vesicuar trafficking in cells.
Sequence similaritiesContains 1 Ras-associating domain.
Cellular localizationEndosome. Accumulates on perinuclear endosomes.
- Information by UniProt
- P CIP1 antibody
- P-CIP1 antibody
- PAM COOH-terminal interactor protein 1 antibody
All lanes : Anti-PAMCI antibody (ab150853) at 1/100 dilution
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
Predicted band size: 50 kDa
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PAMCI with ab150853 at 1/200 dilution
All lanes : Anti-PAMCI antibody (ab150853) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 50 kDa
ab150853 has not yet been referenced specifically in any publications.