Overview

  • Product name

    Anti-PAM/Phenylalanine ammonia-lyase antibody
    See all PAM/Phenylalanine ammonia-lyase primary antibodies
  • Description

    Rabbit polyclonal to PAM/Phenylalanine ammonia-lyase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PAM/Phenylalanine ammonia-lyase aa 338-497.
    Database link: P19021

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human endometrial cancer and heart tissues. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as PAM

     

Properties

Applications

Our Abpromise guarantee covers the use of ab237488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/5000. Predicted molecular weight: 108 kDa.
ICC/IF 1/200 - 1/500.

Target

  • Function

    Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
  • Sequence similarities

    In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
    In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 5 NHL repeats.
  • Cellular localization

    Membrane and Secreted. Secreted from secretory granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMD_HUMAN antibody
    • PAL antibody
    • PAM antibody
    • Pancreatic peptidylglycine alpha amidating monooxygenase antibody
    • Peptidyl alpha amidating enzyme antibody
    • Peptidyl alpha hydroxyglycine alpha amidating lyase antibody
    • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody
    • Peptidylamidoglycolate lyase antibody
    • Peptidylglycine 2 hydroxylase antibody
    • Peptidylglycine alpha amidating monooxygenase antibody
    • Peptidylglycine alpha hydroxylating monooxygenase antibody
    • PHM antibody
    see all

Images

  • Anti-PAM/Phenylalanine ammonia-lyase antibody (ab237488) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 108 kDa

  • Paraffin-embedded human heart tissue stained for PAM/Phenylalanine ammonia-lyase using ab237488 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

     

  • Paraffin-embedded human endometrial cancer tissue stained for PAM/Phenylalanine ammonia-lyase using ab237488 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

     

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PAM/Phenylalanine ammonia-lyase (green) using ab237488 at 1/66 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain was DAPI.

References

ab237488 has not yet been referenced specifically in any publications.

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