Product nameAnti-PAM/Phenylalanine ammonia-lyase antibody [EPR2643(2)]
See all PAM/Phenylalanine ammonia-lyase primary antibodies
DescriptionRabbit monoclonal [EPR2643(2)] to PAM/Phenylalanine ammonia-lyase
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human PAM/Phenylalanine ammonia-lyase (C terminal). The exact sequence is proprietary.
- WB: Human fetal heart tissue lysate and PC-3 cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PAM
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 108 kDa.|
FunctionBifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
Sequence similaritiesIn the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 5 NHL repeats.
Cellular localizationMembrane and Secreted. Secreted from secretory granules.
- Information by UniProt
- AMD_HUMAN antibody
- PAL antibody
- PAM antibody
Anti-PAM/Phenylalanine ammonia-lyase antibody [EPR2643(2)] (ab109175) at 1/10000 dilution (purified) + Human fetal heart tissue lysate at 20 µg
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1%BSA/TBST.
All lanes : Anti-PAM/Phenylalanine ammonia-lyase antibody [EPR2643(2)] (ab109175) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal heart tissue lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 108 kDa
This product has been referenced in:
- Thomsen SK et al. Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic ß-cells. Nat Genet 50:1122-1131 (2018). Read more (PubMed: 30054598) »
- Simpson PD et al. Striking Oxygen Sensitivity of the Peptidylglycine a-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem 290:24891-901 (2015). WB ; Human . Read more (PubMed: 26296884) »