Anti-AKT (phospho T308) antibody (ab38449)
Key features and details
- Rabbit polyclonal to AKT (phospho T308)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-AKT (phospho T308) antibody -
Description
Rabbit polyclonal to AKT (phospho T308) -
Host species
Rabbit -
Specificity
This antibody was made against a peptide directed against the phosphorylated form of AKT1 at T308, but due to a high degree of homology it is predicted to cross react with AKT2 and AKT3 if they are phosphorylated at the corresponding residue.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human AKT aa 250-350 (phospho T308).
Database link: P31749 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Block in 5% non-fat milk in TBST, RT, 90min. In order to detect a clear signal, treatment is required when using this antibody. |
IHC-P | (1) |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 56 kDa. Block in 5% non-fat milk in TBST, RT, 90min. In order to detect a clear signal, treatment is required when using this antibody. |
IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
AKT, also known as protein kinase B (PKB), is a serine/threonine protein kinase. There are three mammalian isoforms of AKT: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation. -
Cellular localization
Cell Membrane, Cytoplasmic and Nuclear. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). -
Database links
- Entrez Gene: 207 Human
- Entrez Gene: 11651 Mouse
- Omim: 164730 Human
- SwissProt: P31749 Human
- SwissProt: P31750 Mouse
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Alternative names
- AKT1 antibody
- C AKT antibody
- PKB antibody
see all
Images
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All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/1000 dilution
Lane 1 : HeLa cells
Lane 2 : HeLa cells treated with 0.01 U/mL Insulin for 15 minutes
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 56 kDa10% gel.
Running conditions: 60v, 30min; 120v 60min
Transfer conditions: 150mA 120min Nitrocellulose membrane.
Blocking conditions: 5% non-fat milk in TBST, RT, 90min.
Primary antibody incubation: 4?, overnight
Secondary antibody incubation: room temperature for 45 minutes
Washing condition: 5 ml TBST, 4 x 5min
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Immunohistochemical analysis of AKT (phospho T308) expression in paraffin embedded human lung carcinoma tissue, using ab38449 (1/50). Right-hand panel represents a negative control where ab38449 was pre-incubated with the immunizing (blocking) peptide.
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All lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/500 dilution
Lane 1 : 293 cell lysate - untreated
Lane 2 : 293 cell lysate - treated with insulin
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (669)
ab38449 has been referenced in 669 publications.
- Li YK et al. Validation of ESM1 Related to Ovarian Cancer and the Biological Function and Prognostic Significance. Int J Biol Sci 19:258-280 (2023). PubMed: 36594088
- Li L et al. Knockdown of miR-92a suppresses the stemness of colorectal cancer cells via mediating SOCS3. Bioengineered 13:5613-5624 (2022). WB ; Human . PubMed: 35184640
- Li W et al. The circ-PITX1 promotes non-small cell lung cancer development via the miR-30e-5p/ITGA6 axis. Cell Cycle 21:304-321 (2022). PubMed: 35007184
- Sun R et al. Pink Lotus Essential Oil and Alleviates on Free Fatty Acid Induced Steatosis in HepG2 Cells via PI3K/Akt and NF-κB Pathways. J Oleo Sci 71:95-104 (2022). PubMed: 35013040
- Kotulova J et al. 2‑Cl‑IB‑MECA regulates the proliferative and drug resistance pathways, and facilitates chemosensitivity in pancreatic and liver cancer cell lines. Int J Mol Med 49:N/A (2022). PubMed: 35039871