Anti-pan Synuclein antibody (ab6176)
Key features and details
- Rabbit polyclonal to pan Synuclein
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-pan Synuclein antibody
See all pan Synuclein primary antibodies -
Description
Rabbit polyclonal to pan Synuclein -
Host species
Rabbit -
Specificity
This antibody recognises all 3 forms of the synucleins. -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis -
Immunogen
Synthetic peptide corresponding to Human pan Synuclein aa 1-100. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Relevance
Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha, beta and gamma synucleins. Synuclein-alpha is primarily localized at the presynaptic region of axons and it may be involved in the regulation of dopamine release and transport. However, it can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Synuclein-alpha may induce fibrillization of the microtubule-associated protein tau and it reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Synuclein-alpha is involved in Parkinson's disease, Lewy body dementia, Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome) and it is a major non-amyloid component of the deposits associated with Alzheimer's disease. Synuclein-beta may be a regulator of the SNCA aggregation process. It protects neurons from staurosporine and 6 hydroxy dopamine (6OHDA)-stimulated caspase activation that also involves p53 and contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Gamma synuclein is found primarily in the peripheral nervous system and retina. High levels of expression have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development. -
Cellular localization
Neuronal cytosol and presynaptic terminals -
Database links
- Entrez Gene: 281494 Cow
- Entrez Gene: 6620 Human
- Entrez Gene: 6622 Human
- Entrez Gene: 6623 Human
- Entrez Gene: 104069 Mouse
- Entrez Gene: 20617 Mouse
- Entrez Gene: 20618 Mouse
- Entrez Gene: 113893 Rat
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Alternative names
- BCSG1 antibody
- beta synuclein antibody
- Breast cancer specific gene 1 protein antibody
see all
Images
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ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab6176 has been referenced in 16 publications.
- Raina A et al. Dopamine promotes the neurodegenerative potential of ß-synuclein. J Neurochem 156:674-691 (2021). PubMed: 32730640
- Dominguez-Meijide A et al. Doxycycline inhibits a-synuclein-associated pathologies in vitro and in vivo. Neurobiol Dis 151:105256 (2021). PubMed: 33429042
- Fouke KE et al. Synuclein Regulates Synaptic Vesicle Clustering and Docking at a Vertebrate Synapse. Front Cell Dev Biol 9:774650 (2021). PubMed: 34901020
- McGlinchey RP et al. C-terminal a-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease. J Biol Chem 294:9973-9984 (2019). PubMed: 31092553
- Mahajani S et al. Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors. Cell Death Dis 10:898 (2019). PubMed: 31776327