Anti-pan-AKT antibody (ab18785)
- Datasheet
- References (14)
- Protocols
Overview
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Product name
Anti-pan-AKT antibody
See all pan-AKT primary antibodies -
Description
Rabbit polyclonal to pan-AKT -
Host species
Rabbit -
Specificity
ab18785 detects Akt1, Akt2 and Akt3. -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Synthetic peptide corresponding to residues surrounding aa 80 of Akt (N terminal)(Human) (Peptide available as ab53312.)
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Positive control
- mouse small intestine tissue lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab18785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | ||
| Flow Cyt | ||
| ICC/IF | ||
| WB | ||
| IP |
Application notes
IHC-Fr: Use at a concentration of 10 - 40 µg/ml.
IP: Use at a concentration of 10 - 40 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 10 - 40 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. -
Tissue specificity
Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. -
Involvement in disease
Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1). -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
The AGC-kinase C-terminal mediates interaction with THEM4. -
Post-translational
modificationsPhosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 207 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 24185 Rat
- Omim: 164730 Human
- SwissProt: P31749 Human
- SwissProt: P31750 Mouse
- SwissProt: P47196 Rat
- Unigene: 525622 Human
see all -
Alternative names
- AKT1 antibody
- AKT1_HUMAN antibody
- AKT2 antibody
see all
Images
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Western blot - Anti-pan-AKT antibody (ab18785)Anti-pan-AKT antibody (ab18785) + Akt expression in NIH3T3 cell lysate
Predicted band size: 56 kDa
Observed band size: 56 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-pan-AKT antibody (ab18785) -
Immunocytochemistry/ Immunofluorescence - Anti-pan-AKT antibody (ab18785)Immunocytochemistry/ Immunofluorescence of HeLa cells staining AKT with ab18785 at 1/10 dilution. Cy3 conjugated Goat anti-rabbit IgG at 1/100 was used a secondary. Counterstained with DAPI.
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Western blot - Anti-pan-AKT antibody (ab18785)Western blot of HeLa cell lysates staining AKT with ab18785
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Flow Cytometry - Anti-pan-AKT antibody (ab18785)Flow cytometry of HeLa cells.
Pink: Primary ab18785 at 1/10. Secondary Goat anti-rabbit IgG Cy3.
Green: Goat anti-rabbit IgG Cy3 only.
Black: Unstained HeLa Cells
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yuan Y & Zheng Z Geniposide protects PC-12 cells against oxygen and glucose deprivation-induced injury by up-regulation of long-noncoding RNA H19. Life Sci 216:176-182 (2019). Read more (PubMed: 30472296) »
- Bao H et al. Astragaloside protects oxygen and glucose deprivation induced injury by regulation of microRNA-21 in retinal ganglion cell line RGC-5. Biomed Pharmacother 109:1826-1833 (2019). Read more (PubMed: 30551437) »
