Synthetic peptide corresponding to residues surrounding aa 80 of Akt (N terminal)(Human) (Peptide available as ab53312.)
Our Abpromise guarantee covers the use of ab18785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunocytochemistry/ Immunofluorescence of HeLa cells staining AKT with ab18785 at 1/10 dilution. Cy3 conjugated Goat anti-rabbit IgG at 1/100 was used a secondary. Counterstained with DAPI.
Western blot of HeLa cell lysates staining AKT with ab18785
Flow cytometry of HeLa cells.
Pink: Primary ab18785 at 1/10. Secondary Goat anti-rabbit IgG Cy3.
Green: Goat anti-rabbit IgG Cy3 only.
Black: Unstained HeLa Cells