Product nameAnti-pan-AKT antibody
See all pan-AKT primary antibodies
DescriptionRabbit polyclonal to pan-AKT
Tested applicationsSuitable for: ICC, IHC-P, WB, ICC/IF, IHC-Fr, Flow Cyt, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
- WB: NIH/3T3 whole cell lysate IHC: colon tumour sections
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Note: 0.02M Potassium phosphate, 0.15M Sodium chloride
Our Abpromise guarantee covers the use of ab8805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/1500.|
|WB||1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24759991|
|Flow Cyt||Use at an assay dependent concentration.|
|ELISA||1/2000 - 1/10000.|
FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
The AGC-kinase C-terminal mediates interaction with THEM4.
modificationsPhosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
- Information by UniProt
- AKT1 antibody
- AKT1_HUMAN antibody
- AKT2 antibody
Western blot using Akt antibody ab8805 at 1/500, Goat anti-rabbit IgG peroxidase conjugate at 1/10,000. 20 ug NIH/3T3 whole cell lysate, Gel type 10% NuPage with MOPS buffer developed with Substrate Pierce SuperSignal™ West PicoWestern blot using Akt antibody ab8805 at 1/500, Goat anti-rabbit IgG peroxidase conjugate at 1/10,000. 20 ug NIH/3T3 whole cell lysate, Gel type 10% NuPage with MOPS buffer developed with Substrate Pierce SuperSignal™ West Pico
The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.
A: Normal colon tissue
B: Tumour tissue
Akt antibody (ab8805) used at 1/1000 on formalin-fixed paraffin embedded tissue.
Anti-pan-AKT antibody (ab8805) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
This product has been referenced in:
- Gao JX et al. Overexpression of microRNA-183 promotes apoptosis of substantia nigra neurons via the inhibition of OSMR in a mouse model of Parkinson's disease. Int J Mol Med 43:209-220 (2019). Read more (PubMed: 30431059) »
- Zhang H et al. Crocetin inhibits PDGF-BB-induced proliferation and migration of retinal pigment epithelial cells. Eur J Pharmacol 842:329-337 (2019). Read more (PubMed: 30395849) »