Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Arrestin antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293)
Specification
HEK 293
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Other product details

Dilution
1/75
Incubation time
18 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Flour 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The cells were fixed in 4% PFA (in PBS) for 30min. After two PBS washes (each 5min) the cells were permeabilised with 0.01% Triton X-100 in PBS (PBS-T) for 1h at RT. The cells were incubated with the primary antibody in PBS-T for 18h at 4C. After three 5min washes (PBS) the cells were incubated for 1h at RT with the secondary antibody. After three 5min washes with PBS the cover slips were mounted with slow fade mounting solution containing DAPI (Molecular Probes) .
Figure legend: Dapi(blue) nuclear staining, beta-arrestin (green)

Mrs. Danijela Markovic

Verified customer

Submitted Aug 30 2006

Sign up