Product nameAnti-pan Cadherin antibody
See all pan Cadherin primary antibodies
DescriptionRabbit polyclonal to pan Cadherin
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Electron Microscopy, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Zebrafish
Predicted to work with: Rabbit, Bird, Fish, Amphibian
General notesImmunohistochemistry This antibody has not been tested on frozen sections but has been tested on both cultured cells and formalin-fixed, paraffin-embedded, protease digested sections so chances are it will work on frozen sections. It has also been tested on heart sections and MDBK cells. This antibody should work on bovine samples although we have not tested it specifically.
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Primary antibody notesThis product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
Our Abpromise guarantee covers the use of ab6529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|WB||1/200. On chicken and rabbit heart extract this antibody detects a single band at 135 kD.|
|ICC/IF||1/100. Fix cells with 2-4% paraformaldehyde for 10 minutes at room temperature, followed by 10-minute incubation with 0.2% Triton X-100 (see Gellersen et al 2007 and Kaur et al 2006).|
|Electron Microscopy||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. Fix with ice cold ethanol.|
RelevanceCadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
Immunofluorescent imaging of human cells (U2OS) with ab6529 confirms the specificity of this antibody. Pan cadherin antibody recognises predominantly membranous signal with some fainter cytoplasmic staining corresponding to soluble cadherin. No nuclear staining is seen.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.
ab6529 staining pan Cadherin in human bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted, 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200 was used as secondary.
All Lanes: pan Cadherin antibody (ab6529) at 1/500 dilution + Mouse brain whole tissue lysate (100 µg)
Secondary Antibody: An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal developed using the ECL technique
Blocking Step: 5% Milk for 1 hour at 25 °C.
Gel Running Conditions: Reduced, denaturing.
ab6529 staining pan Cadherin in rat kidney tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/200 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.
ab6529 staining pan Cadherin in murine liver cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.4% Triton X-100 and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 17 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution. The other secondary antibody used was an Alexa Fluor® 488 (green) goat anti-mouse IgG for detection of a specific cytosolic protein.
This product has been referenced in:
- Choi EK et al. Functional analysis of SLC39A8 mutations and their implications for manganese deficiency and mitochondrial disorders. Sci Rep 8:3163 (2018). Read more (PubMed: 29453449) »
- Higgins SJ et al. Tie2 protects the vasculature against thrombus formation in systemic inflammation. J Clin Invest 128:1471-1484 (2018). Read more (PubMed: 29360642) »