Overview

  • Product name
    Anti-pan Cadherin antibody
    See all pan Cadherin primary antibodies
  • Description
    Rabbit polyclonal to pan Cadherin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Electron Microscopy, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Zebrafish
    Predicted to work with: Rabbit, Bird, Fish, Amphibian
  • Immunogen

    Synthetic peptide:

    DYDYLNDWGPRFKKLADMYGGGDD

    conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 889-912 of Chicken pan Cadherin.

  • General notes
    Immunohistochemistry This antibody has not been tested on frozen sections but has been tested on both cultured cells and formalin-fixed, paraffin-embedded, protease digested sections so chances are it will work on frozen sections. It has also been tested on heart sections and MDBK cells. This antibody should work on bovine samples although we have not tested it specifically.


    This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Properties

Applications

Our Abpromise guarantee covers the use of ab6529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB 1/200. On chicken and rabbit heart extract this antibody detects a single band at 135 kD.
ICC/IF 1/100. Fix cells with 2-4% paraformaldehyde for 10 minutes at room temperature, followed by 10-minute incubation with 0.2% Triton X-100 (see Gellersen et al 2007 and Kaur et al 2006).
Electron Microscopy Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with ice cold ethanol.

Target

Images

  • Immunofluorescent imaging of human cells (U2OS) with ab6529 confirms the specificity of this antibody.  Pan cadherin antibody recognises predominantly membranous signal with some fainter cytoplasmic staining corresponding to soluble cadherin. No nuclear staining is seen. 

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.

  • ab6529 staining pan Cadherin in human bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted, 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200 was used as secondary.

  • All Lanes: pan Cadherin antibody (ab6529) at 1/500 dilution + Mouse brain whole tissue lysate (100 µg)

    Secondary Antibody: An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal developed using the ECL technique

    Blocking Step: 5% Milk for 1 hour at 25 °C.

    Gel Running Conditions: Reduced, denaturing.

    See Abreview

  • ab6529 staining pan Cadherin in rat kidney tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/200 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

    See Abreview

  • ab6529 staining pan Cadherin in murine liver cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.4% Triton X-100 and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 17 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution. The other secondary antibody used was an Alexa Fluor® 488 (green) goat anti-mouse IgG for detection of a specific cytosolic protein.

    See Abreview

References

This product has been referenced in:
  • Choi EK  et al. Ferroportin disease mutations influence manganese accumulation and cytotoxicity. FASEB J 33:2228-2240 (2019). Read more (PubMed: 30247984) »
  • Ip JPK  et al. Major Vault Protein, a Candidate Gene in 16p11.2 Microdeletion Syndrome, Is Required for the Homeostatic Regulation of Visual Cortical Plasticity. J Neurosci 38:3890-3900 (2018). Read more (PubMed: 29540554) »
See all 30 Publications for this product

Customer reviews and Q&As

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1-10 of 14 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Roti­-ImmunoBlock as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 26°C
Sample
Cat Cell (CrFK)
Specification
CrFK
Permeabilization
Yes - Triton X-100 0.2 %
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 27 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver (20 um section))
Specification
Liver (20 um section)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 18 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2)
Specification
HepG2
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 29 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (liver)
Specification
liver
Fixative
Formaldehyde
Permeabilization
Yes - 0.4% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Hang Zhao

Verified customer

Submitted Jan 26 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Kidney)
Specification
Kidney
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 24 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H1299)
Specification
H1299
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 11 2010

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
100 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293 FT cell line)
Specification
293 FT cell line
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 21 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Smooth muscle)
Specification
Smooth muscle
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 04 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (bone marrow cell)
Specification
bone marrow cell
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 24 2009

1-10 of 14 Abreviews

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