Recombinant Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-396924-anti-pan-cadherin-antibody-epr1792y-westernblot-human-mouse-rat.jpg "Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
See all pan Cadherin primary antibodies -
Description
Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
Database link: P19022 -
Positive control
- WB: C6 whole cell lysate. Mouse heart tissue lysate. Human, mouse, and rat brain tissue lysates; ICC: MCF7 cells; IHC-P: Rat, mouse and human cardiac muscle tissue; human and rat colon and mouse testis tissue; IP: Rat heart lysate; Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 6.10 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1792Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
For unpurified use at 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa).
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| IP |
1/20.
For unpurified use at 1/40. |
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| IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100. |
| ICC/IF | (2) |
1/100 - 1/250.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/20. For unpurified use at 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa). |
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IP
1/20. For unpurified use at 1/40. |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100. |
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ICC/IF
1/100 - 1/250. |
Target
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Relevance
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. -
Database links
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
Alternative names
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
Images
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Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)All lanes : Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified)
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : Human brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-15-anti-pan-cadherin-antibody-epr1792y-immunohistochemistry-cardiac-muscle-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354834-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunocytochemistry-immunofluorescence-human-mcf7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cells labeling pan Cadherin with purified ab51034 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as counterstain antibody.
Confocal image showing membranous staining in MCF7 cells.
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![Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-10-anti-pan-cadherin-antibody-epr1792y-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling pan Cadherin with purified ab51034 at 1/20 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-9-anti-pan-cadherin-antibody-epr1792y-immunoprecipitation-heart-rat.jpg)
Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Purified ab51034 at 1/20 dilution (0.6 µg) immunoprecipitating pan Cadherin in Rat heart lysate.
Lane 1 (input): Rat heart lysate 10 µg
Lane 2 (+): ab51034 + Rat heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51034 in Rat heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 140 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-14-anti-pan-cadherin-antibody-epr1792y-immunohistochemistry-cardiac-muscle-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-13-anti-pan-cadherin-antibody-epr1792y-immunohistochemistry-cardiac-muscle-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354835-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemical analysis of Paraffin-embedded human colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on human colon.
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![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-396922-anti-pan-cadherin-antibody-epr1792y-westernblot-heart-mouse.jpg)
Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified) + Mouse heart lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354837-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunohistochemistry-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemical analysis of Paraffin-embedded rat colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on rat colon.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354836-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemical analysis of Paraffin-embedded mouse testis tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on mouse testis.
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![OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-62886-anti-pan-cadherin-antibody-epr1792y-intercellular-junction-marker.jpg)
OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) -
![Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-16-benefits-of-recombinant-antibodies.png)
Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab51034 has been referenced in 15 publications.
- Bai S et al. Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-?B/MMP-2 axis. Cell Death Dis 11:1008 (2020). PubMed: 33230102
- Chen W et al. Blocking interleukin-6 trans-signaling protects against renal fibrosis by suppressing STAT3 activation. Theranostics 9:3980-3991 (2019). PubMed: 31281526
- Zhang C et al. Sulforaphene induces apoptosis and inhibits the invasion of esophageal cancer cells through MSK2/CREB/Bcl-2 and cadherin pathway in vivo and in vitro. Cancer Cell Int 19:342 (2019). PubMed: 31889894
- Chung TW et al. Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells. Onco Targets Ther 12:6119-6131 (2019). PubMed: 31496721
- Yang J et al. Notch signaling is important for epithelial-mesenchymal transition induced by low concentrations of doxorubicin in osteosarcoma cell lines. Oncol Lett 13:2260-2268 (2017). PubMed: 28454389