Recombinant
RabMAb

Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

Overview

  • Product name
    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
    See all pan Cadherin primary antibodies
  • Description
    Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
    Database link: P19022

  • Positive control
    • C6 cell lysate, human cervical carcinoma tissue or HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa).
IP 1/40.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

Images

  • Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution + C6 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?

  • Ab51034 (1/100) staining N Cadherin in human cervical carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
  • Immunofluorescent staining of HeLa cells using ab51034 (1/100).
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Yang J  et al. Notch signaling is important for epithelial-mesenchymal transition induced by low concentrations of doxorubicin in osteosarcoma cell lines. Oncol Lett 13:2260-2268 (2017). Read more (PubMed: 28454389) »
  • Zou L  et al. Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa). PLoS One 12:e0179421 (2017). WB . Read more (PubMed: 28614361) »
See all 11 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Jun 13 2018

Application
Western blot
Sample
Rat Cell lysate - other (Rat hepatocytes - palsma membrane)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes - palsma membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Jun 01 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
Yes - 0.1% Tween 20 in PBS
Specification
kidney
Blocking step
Serum as blocking agent for 25 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Miss. Chia-Li Lin

Verified customer

Submitted Dec 03 2015

Question
Answer

Thank you for contacting us.

The concentration for lot GR73907-3 is 0.048 mg/mL.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting Abcam. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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