Recombinant Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
- Datasheet
- References (12)
- Protocols
Overview
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Product name
Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
See all pan Cadherin primary antibodies -
Description
Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
Database link: P19022 -
Positive control
- C6 cell lysate, human cervical carcinoma tissue or HeLa cells, MCF7 cells. IHC-P: Human and rat colon and mouse testis tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 6.10 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1792Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa). | |
| IP | 1/40. | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Relevance
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. -
Database links
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
Alternative names
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
Images
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![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-62889-anti-pan-cadherin-antibody-epr1792y-intercellular-junction-marker-western-blot.jpg)
Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution + C6 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354835-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemical analysis of Paraffin-embedded human colon tissue sections labeling pan Cadherin with ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on human colon.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354834-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunocytochemistry-immunofluorescence-human-mcf7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cells labeling pan Cadherin with ab51034 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as counterstain antibody.
Confocal image showing membranous staining in MCF7 cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354837-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunohistochemistry-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemical analysis of Paraffin-embedded rat colon tissue sections labeling pan Cadherin with ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on rat colon.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-354836-antipan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunohistochemical analysis of Paraffin-embedded mouse testis tissue sections labeling pan Cadherin with ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on mouse testis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-62888-anti-pan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Ab51034 (1/100) staining N Cadherin in human cervical carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-62887-anti-pan-cadherin-antibody-epr1792y-intercellular-junction-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunofluorescent staining of HeLa cells using ab51034 (1/100). -
![Other - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034-62886-anti-pan-cadherin-antibody-epr1792y-intercellular-junction-marker.jpg)
Other - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
References
This product has been referenced in:
- Chen W et al. Blocking interleukin-6 trans-signaling protects against renal fibrosis by suppressing STAT3 activation. Theranostics 9:3980-3991 (2019). Read more (PubMed: 31281526) »
- Yang J et al. Notch signaling is important for epithelial-mesenchymal transition induced by low concentrations of doxorubicin in osteosarcoma cell lines. Oncol Lett 13:2260-2268 (2017). Read more (PubMed: 28454389) »
