Recombinant
Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
- Datasheet
- References (11)
- Protocols
Overview
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Product nameAnti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
See all pan Cadherin primary antibodies -
DescriptionRabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
Database link: P19022 -
Positive control
- C6 cell lysate, human cervical carcinoma tissue or HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Dissociation constant (KD)KD = 6.10 x 10 -10 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR1792Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa). | |
| IP | 1/40. | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
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RelevanceCadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
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Database links
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
Alternative names
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
Images
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![Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034_1.jpg)
Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution + C6 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Ab51034 (1/100) staining N Cadherin in human cervical carcinoma tissue by immunohistochemistry using paraffin embedded tissue. -
![Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/ab51034_3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)Immunofluorescent staining of HeLa cells using ab51034 (1/100). -
![Other - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)](https://www.abcam.com/ps/products/51/ab51034/Images/pan-Cadherin-Primary-antibodies-ab51034-1.jpg)
Other - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
References
This product has been referenced in:
- Yang J et al. Notch signaling is important for epithelial-mesenchymal transition induced by low concentrations of doxorubicin in osteosarcoma cell lines. Oncol Lett 13:2260-2268 (2017). Read more (PubMed: 28454389) »
- Zou L et al. Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa). PLoS One 12:e0179421 (2017). WB . Read more (PubMed: 28614361) »
