Recombinant
RabMAb

Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

Overview

  • Product name
    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
    See all pan Cadherin primary antibodies
  • Description
    Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
    Database link: P19022

  • Positive control
    • C6 cell lysate, human cervical carcinoma tissue or HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa).
IP 1/40.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

Images

  • Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution + C6 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 140 kDa (why is the actual band size different from the predicted?)

  • Ab51034 (1/100) staining N Cadherin in human cervical carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
  • Immunofluorescent staining of HeLa cells using ab51034 (1/100).
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Yang J  et al. Notch signaling is important for epithelial-mesenchymal transition induced by low concentrations of doxorubicin in osteosarcoma cell lines. Oncol Lett 13:2260-2268 (2017). Read more (PubMed: 28454389) »
  • Zou L  et al. Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa). PLoS One 12:e0179421 (2017). WB . Read more (PubMed: 28614361) »

See all 11 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jun 13 2018

Application
Western blot
Sample
Rat Cell lysate - other (Rat hepatocytes - palsma membrane)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes - palsma membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jun 01 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
Yes - 0.1% Tween 20 in PBS
Specification
kidney
Blocking step
Serum as blocking agent for 25 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Miss. Chia-Li Lin

Verified customer

Submitted Dec 03 2015

Thank you for contacting us.

The concentration for lot GR73907-3 is 0.048 mg/mL.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please cont...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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