RecombinantRabMAb

Recombinant Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

Reviews (6)Q&A (2)References (15)
Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
  • Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker
    See all pan Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR1792Y] to pan Cadherin - Intercellular Junction Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human pan Cadherin (C terminal). The exact sequence is proprietary.
    Database link: P19022

  • Positive control

    • WB: C6 whole cell lysate. Mouse heart tissue lysate. Human, mouse, and rat brain tissue lysates; ICC: MCF7 cells; IHC-P: Rat, mouse and human cardiac muscle tissue; human and rat colon and mouse testis tissue; IP: Rat heart lysate; Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

For unpurified use at 1/30.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa).
IP
1/20.

For unpurified use at 1/40.
IHC-P (2)
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.
ICC/IF (2)
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/20.

For unpurified use at 1/30.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa).
IP
1/20.

For unpurified use at 1/40.
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.
ICC/IF
1/100 - 1/250.

Target

Images

  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    All lanes : Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified)

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : Human brain lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cells labeling pan Cadherin with purified ab51034 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. 

    ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as counterstain antibody.

    Confocal image showing membranous staining in MCF7 cells.

  • Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Flow Cytometry (Intracellular) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling pan Cadherin with purified ab51034 at 1/20 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunoprecipitation - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Purified ab51034 at 1/20 dilution (0.6 µg) immunoprecipitating pan Cadherin in Rat heart lysate.
    Lane 1 (input): Rat heart lysate 10 µg
    Lane 2 (+): ab51034 + Rat heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51034 in Rat heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling pan Cadherin with purified ab51034 at 1/500 dilution (0.26 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded human colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on human colon.

  • Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Western blot - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034) at 1/50000 dilution (purified) + Mouse heart lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded rat colon tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on rat colon.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

    Immunohistochemical analysis of Paraffin-embedded mouse testis tissue sections labeling pan Cadherin with unpurified ab51034 at 1/100. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on mouse testis.

  • OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    OI-RD Scanning - Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)
    Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker (ab51034)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (15)

Publishing research using ab51034? Please let us know so that we can cite the reference in this datasheet.

ab51034 has been referenced in 15 publications.

  • Bai S  et al. Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-?B/MMP-2 axis. Cell Death Dis 11:1008 (2020). PubMed: 33230102
  • Chen W  et al. Blocking interleukin-6 trans-signaling protects against renal fibrosis by suppressing STAT3 activation. Theranostics 9:3980-3991 (2019). PubMed: 31281526
  • Zhang C  et al. Sulforaphene induces apoptosis and inhibits the invasion of esophageal cancer cells through MSK2/CREB/Bcl-2 and cadherin pathway in vivo and in vitro. Cancer Cell Int 19:342 (2019). PubMed: 31889894
  • Chung TW  et al. Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells. Onco Targets Ther 12:6119-6131 (2019). PubMed: 31496721
  • Yang J  et al. Notch signaling is important for epithelial-mesenchymal transition induced by low concentrations of doxorubicin in osteosarcoma cell lines. Oncol Lett 13:2260-2268 (2017). PubMed: 28454389
View all Publications for this product

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human bone marrow derived stromal cells transplant)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Human bone marrow derived stromal cells transplant
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted May 26 2020

Immunocytochemistry abreview for Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker

 Good
Abreviews
Application
Immunocytochemistry
Sample
Human Cultured Cells (Cultured human bone marrow derived stromal cells)
Permeabilization
Yes - 0.2% Triton, 30min RT
Specification
Cultured human bone marrow derived stromal cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Dr. Queralt Vallmajó

Verified customer

Submitted Apr 02 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
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Dr. Armen Petrosyan

Verified customer

Submitted Nov 20 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

Submitted Jun 13 2018

Western blot abreview for Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - other (Rat hepatocytes - palsma membrane)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes - palsma membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Armen Petrosyan

Verified customer

Submitted Jun 01 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cadherin antibody [EPR1792Y] - Intercellular Junction Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
Yes - 0.1% Tween 20 in PBS
Specification
kidney
Blocking step
Serum as blocking agent for 25 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Miss. Chia-Li Lin

Verified customer

Submitted Dec 03 2015

Question

Please send the concentration of lot GR73907-3.

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Answer

Thank you for contacting us.

The concentration for lot GR73907-3 is 0.048 mg/mL.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I would like a replacement with ab71055.

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Answer

Thank you for contacting Abcam. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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