For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    pan-cadherin-antibody-mabcam22744-ab22744.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Plasma Membrane
Share by email

Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

  • Datasheet
  • SDS
Reviews (13)Q&A (6)References (18)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

Key features and details

  • Mouse monoclonal [mAbcam22744] to pan Cadherin
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

HRP

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
Product image
Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
Primary
Anti-BCRP/ABCG2 antibody [BXP-21] (ab3380)

View more associated products

Overview

  • Product name

    Anti-pan Cadherin antibody [mAbcam22744]
    See all pan Cadherin primary antibodies
  • Description

    Mouse monoclonal [mAbcam22744] to pan Cadherin
  • Host species

    Mouse
  • Specificity

    Detects a weaker band in human heart than in rat heart.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Dog, Xenopus laevis, Monkey, Zebrafish, African green monkey
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    IgG fraction
  • Primary antibody notes

    This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
  • Clonality

    Monoclonal
  • Clone number

    mAbcam22744
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins

Associated products

  • Alternative Versions

    • HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab22744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (4)
Use a concentration of 5 µg/ml.
WB (6)
1/1000. Detects a band of approximately 125-140 kDa (predicted molecular weight: 125 kDa).

Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below.

Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/1000. Detects a band of approximately 125-140 kDa (predicted molecular weight: 125 kDa).

Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below.

Application notes
Is unsuitable for Flow Cyt.

Target

  • Relevance

    Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
  • Database links

    • Entrez Gene: 1001 Human
    • Entrez Gene: 1002 Human
    • Entrez Gene: 1003 Human
    • Entrez Gene: 1004 Human
    • Entrez Gene: 1005 Human
    • Entrez Gene: 1006 Human
    • Entrez Gene: 1008 Human
    • Entrez Gene: 1009 Human
    • Entrez Gene: 1012 Human
    • Entrez Gene: 1014 Human
    • Entrez Gene: 1015 Human
    • Entrez Gene: 999 Human
    • Omim: 114020 Human
    • Omim: 114021 Human
    • Omim: 192090 Human
    • Omim: 601120 Human
    • Omim: 603006 Human
    • SwissProt: P12830 Human
    • SwissProt: P19022 Human
    • SwissProt: P22223 Human
    • SwissProt: P33151 Human
    • SwissProt: P55283 Human
    • SwissProt: P39038 Mouse
    see all
  • Alternative names

    • Cadherin antibody
    • CDH3 antibody
    • CDHP antibody
    • 7B4 antigen antibody
    • Cadherin-1 antibody
    • Cadherin-2 antibody
    • Cadherin-3 antibody
    • Cadherin-4 antibody
    • Cadherin-5 antibody
    • CAM 120/80 antibody
    • CD144 antibody
    • CD324 antibody
    • CD325 antibody
    • Cdh4 antibody
    • CDH5 antibody
    • CDHE antibody
    • CDHN antibody
    • E-cadherin antibody
    • Epithelial cadherin antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • P-cadherin antibody
    • Placental cadherin antibody
    • UVO antibody
    • Uvomorulin antibody
    • Vascular endothelial cadherin antibody
    see all

Images

  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Lane 1 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% BSA)
    Lane 2 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% Milk)

    All lanes : Heart (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 125 kDa


    Exposure time: 30 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

    ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    All lanes : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate (blocked with 5% Milk)
    Lane 2 : Heart (Mouse) Tissue Lysate (blocked with 5% Milk)
    Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 5% Milk)
    Lane 4 : Heart (Rat) Tissue Lysate (blocked with 3% Milk)
    Lane 5 : Heart (Mouse) Tissue Lysate (blocked with 3% Milk)
    Lane 6 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 3% Milk)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 125 kDa
    Observed band size: 125 kDa
    Additional bands at: 25 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)Image courtesy of an anonymous Abreview.
    ab22744 staining pan Cadherin in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol, permeabilised in 0.5% (w/v) saponin and then blocked using 10% serum for 2 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 2 hours at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. Counterstained with DAPI (blue).

    See Abreview

  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)This image is courtesy of an Anonymous abreview.
    Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1/500 dilution + Mouse cultured cortical neurons at 20 µg

    Secondary
    HRP-conjugated Goat Anti-Mouse IgG (H+L) polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 125 kDa


    Exposure time: 1 minute


    Blocking performed with 5% milk for 1 hour.
    Primary diluted with PBS + 0.5% Tween20 and incubated for 12 hours at 4°C
    Performed under denaturing conditions.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

    ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (18)

Publishing research using ab22744? Please let us know so that we can cite the reference in this datasheet.

ab22744 has been referenced in 18 publications.

  • Kong LR  et al. A common MET polymorphism harnesses HER2 signaling to drive aggressive squamous cell carcinoma. Nat Commun 11:1556 (2020). PubMed: 32214092
  • Joksimovic SL  et al. Selective inhibition of CaV3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain. Sci Signal 11:N/A (2018). PubMed: 30154101
  • Storman EM  et al. Physical Linkage of Estrogen Receptor a and Aromatase in Rat: Oligocrine and Endocrine Actions of CNS-Produced Estrogens. Endocrinology 159:2683-2697 (2018). PubMed: 29771302
  • Deng Q  et al. Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells. Int J Endocrinol 2018:4537214 (2018). PubMed: 29686703
  • Zemel BM  et al. Calcineurin Dysregulation Underlies Spinal Cord Injury-Induced K+ Channel Dysfunction in DRG Neurons. J Neurosci 37:8256-8272 (2017). PubMed: 28751455
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 19 Abreviews or Q&A

Western blot abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (7%)
Loading amount
10 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Read More

DR. Junmo Hwang

Verified customer

Submitted Jun 28 2016

Western blot abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Cortex)
Gel Running Conditions
Reduced Denaturing (7%)
Loading amount
30 µg
Specification
Cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Read More

DR. Junmo Hwang

Verified customer

Submitted Mar 11 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (J774A.1 macrophages)
Permeabilization
Yes - 0.05% saponin
Specification
J774A.1 macrophages
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

Submitted Jul 15 2015

IHC - Wholemount abreview for Anti-pan Cadherin [mAbcam22744] antibody - Plasma Membrane Marker

Poor
Abreviews
Abreviews
Application
IHC - Wholemount
Sample
Zebrafish Embryo (whole embryo staining)
Specification
whole embryo staining
Read More

Abcam user community

Verified customer

Submitted Jun 10 2014

Western blot abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Dec 06 2012

Immunohistochemistry (Frozen sections) abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain, Spinal cord)
Specification
Brain, Spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Dec 08 2011

Question

Product code: 22744
Lot number: GR64651-1

Inquiry: Problem: Multiple bands detected (see attached file) Sample: Human colon adenocarcinoma cell line HCT-116 Specification: Whole cell lysate Amount of protein loaded: 10 ug Electrophoresis conditions: Reducing and denaturing 10% SDS-PAGE Blocking conditions: 5% milk in TBS-T for 2 hr, room temperature Primary antibody conditions: 1:5000 in 2% BSA in TBS-T, 2 hr, room temperature Secondary antibody: Non-Abcam Sheep anti-mouse HRP Secondary antibody conditions: 1:5000 in 2% BSA in TBS-T, 1 hr, room temperature Detection: DURA (Pierce), 3 min Wash steps: 3 x 5 min using TBS-T

Read More

Abcam community

Verified customer

Asked on Jun 27 2012

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab22744 is tested and covered by our 6 month guarantee for use in WB and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab22744.

1.) I suggest to not use Tween in the blocking solution. The detergent could mask proteins that should be blocked and therefore allow the background bands. Other users have good experience with blocking over night .

2.) To achieve the most specific binding of the antibody I recommend to incubate it at 4C over night.

3.) Maybe the addition of DTT was reducing agent instead of beta mercaptoethanol will help reduce the background..

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More

Abcam Scientific Support

Answered on Jun 27 2012

Question

Hi, Could you recommend a good antibody that I could use as loading control for western blot on cell membrane fractions? Thanks

Read More

Abcam community

Verified customer

Asked on Mar 30 2012

Answer

Thank you for your enquiry.

Below is a selection of antibodies that are suitable for detecting the plasma membrane and nuclear envelope. They all work in western blot and I would review the datasheets to confirm which species they are tested against.

Plasma membrane
ab4645 Anti-Pma1 antibody [40B7]
www.abcam.com/4645

ab7671 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
www.abcam.com/ab7671

ab16505 Anti-pan Cadherin antibody
www.abcam.com/ab16505

ab22744 Anti-pan Cadherin antibody [mAbcam22744]
www.abcam.com/ab22744

Nuclear envelope
ab8980 Anti-Lamin A antibody [133A2]
www.abcam.com/ab8980

ab8984 Anti-Lamin A + C antibody [131C3]
www.abcam.com/ab8984

ab16048 Anti-Lamin B1 antibody
www.abcam.com/ab16048

I hope this is useful. Just let me know if you have further questions.

Read More

Abcam Scientific Support

Answered on Mar 30 2012

Question

Thanks for your prompt reply. I will use methanol to fix my cells and try cadherin antibody again. I will let you know the results in a few days.

Read More

Abcam community

Verified customer

Asked on Mar 28 2012

Answer

OK, good luck. If it does not work, I will have some suggestions for a different membrane marker, most likely anti-Na/K ATPase.

Read More

Abcam Scientific Support

Answered on Mar 28 2012

Question

I have tested the new cadherin antibody (ab22744). It works better than the previous one. However, it is not what I expected to be. Here attached the images.

I followed the suggested concentration (5 ug/ml) and general protocol for immunocytochemistry. I also used a higher concentration (10 ug/ml). The problem is not because of the cadherin concentration but the specificity to the cadherin as a plasma membrane marker within my Sol8 cells (a myogenic cell line).

I am thinking of using another plasma membrane marker. How about the Na/K ATPase? I wish I can get another replacement.

I will look forward to your reply. Thanks for your time and help.

Read More

Abcam community

Verified customer

Asked on Mar 27 2012

Answer

Thank you for the images of the staining with ab22744.

I agree the staining is not what is usually observed for cadherin. I think you are expecting something like what one of our reviewers saw. The data is at this link:

https://www.abcam.com/pan-Cadherin-antibody-mAbcam22744-ab22744.html&tab=abreviews&intabreviewid=22768

Are you still fixing your cells with PFA? It may be that correct staining is dependent on the fixation protocol. The data we have for ab22744 is all from methanol-fixed cells. I suggest trying this if you are able to, fixation in cold methanol for five minutes, followed by rinsing with PBS.

Please let me know if you are willing to try this. If not, we can discuss replacement with a Na ATPase antibody.

Read More

Abcam Scientific Support

Answered on Mar 27 2012

1-10 of 19 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.