Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
- Datasheet
- Specific References (13)
- Protocols
Overview
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Product nameAnti-pan Cadherin antibody [mAbcam22744]
See all pan Cadherin primary antibodies -
DescriptionMouse monoclonal [mAbcam22744] to pan Cadherin
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Host speciesMouse
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SpecificityDetects a weaker band in human heart than in rat heart.
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human, African green monkey
Predicted to work with: Chicken, Dog, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide at the C-terminus of Human Cadherins.
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General notes
This antibody clone is manufactured by Abcam.
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.50
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
PurityIgG fraction
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Primary antibody notesThis product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
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ClonalityMonoclonal
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Clone numbermAbcam22744
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab22744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/1000. Detects a band of approximately 125-140 kDa (predicted molecular weight: 125 kDa). Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below. |
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| IHC-P | 1/2000. |
Target
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RelevanceCadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
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Database links
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
Alternative names
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
Images
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![Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)](https://www.abcam.com/ps/products/22/ab22744/Images/pan-Cadherin-Primary-antibodies-ab22744-33.jpg)
Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)Lane 1 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% BSA)
Lane 2 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% Milk)
All lanes : Heart (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 125 kDa
Exposure time: 30 seconds -
![Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)](https://www.abcam.com/ps/products/22/ab22744/Images/ab22744-IF-Im1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
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![Flow Cytometry - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)](https://www.abcam.com/ps/products/22/ab22744/Images/pan-Cadherin-Primary-antibodies-ab22744-41.jpg)
Flow Cytometry - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)Overlay histogram showing HEK293 cells stained with ab22744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22744, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)](https://www.abcam.com/ps/products/22/ab22744/Images/ab22744-200031-WB1AP10105921.jpg)
Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)All lanes : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate (blocked with 5% Milk)
Lane 2 : Heart (Mouse) Tissue Lysate (blocked with 5% Milk)
Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 5% Milk)
Lane 4 : Heart (Rat) Tissue Lysate (blocked with 3% Milk)
Lane 5 : Heart (Mouse) Tissue Lysate (blocked with 3% Milk)
Lane 6 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 3% Milk)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
Observed band size: 125 kDa
Additional bands at: 25 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
![Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)](https://www.abcam.com/ps/products/22/ab22744/Images/pan-Cadherin-Primary-antibodies-ab22744-34.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)Image courtesy of an anonymous Abreview.ab22744 staining pan Cadherin in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol, permeabilised in 0.5% (w/v) saponin and then blocked using 10% serum for 2 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 2 hours at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. Counterstained with DAPI (blue). -
![Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)](https://www.abcam.com/ps/products/22/ab22744/Images/pan-Cadherin-Primary-antibodies-ab22744-27.jpg)
Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)This image is courtesy of an Anonymous abreview.Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1/500 dilution + Mouse cultured cortical neurons at 20 µg
Secondary
HRP-conjugated Goat Anti-Mouse IgG (H+L) polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
Exposure time: 1 minute
Blocking performed with 5% milk for 1 hour.
Primary diluted with PBS + 0.5% Tween20 and incubated for 12 hours at 4°C
Performed under denaturing conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)](https://www.abcam.com/ps/products/22/ab22744/Images/ab22744-IF-Im2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
Protocols
References
This product has been referenced in:
- Deng Q et al. Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells. Int J Endocrinol 2018:4537214 (2018). Read more (PubMed: 29686703) »
- Xia Z et al. Zebrafish slc30a10 deficiency revealed a novel compensatory mechanism of Atp2c1 in maintaining manganese homeostasis. PLoS Genet 13:e1006892 (2017). ICC/IF ; Zebrafish . Read more (PubMed: 28692648) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"