For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    pan-cadherin-antibody-mabcam22744-ab22744.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Plasma Membrane
Share by email

Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

  • Datasheet
  • SDS
Reviews (13)Q&A (6)References (16)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Flow Cytometry - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

Key features and details

  • Mouse monoclonal [mAbcam22744] to pan Cadherin
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
Product image
Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
Primary
Anti-BCRP/ABCG2 antibody [BXP-21] (ab3380)

View more associated products

Overview

  • Product name

    Anti-pan Cadherin antibody [mAbcam22744]
    See all pan Cadherin primary antibodies
  • Description

    Mouse monoclonal [mAbcam22744] to pan Cadherin
  • Host species

    Mouse
  • Specificity

    Detects a weaker band in human heart than in rat heart.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human pan Cadherin aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • General notes

    This antibody clone is manufactured by Abcam.

    This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    IgG fraction
  • Primary antibody notes

    This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
  • Clonality

    Monoclonal
  • Clone number

    mAbcam22744
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins

Associated products

  • Alternative Versions

    • HRP Anti-pan Cadherin antibody [mAbcam22744] (ab197725)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab22744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Rat
All applications
Chicken
Dog
Xenopus laevis
Monkey
Zebrafish
African green monkey
Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF (4)
Use a concentration of 5 µg/ml.
WB (6)
1/1000. Detects a band of approximately 125-140 kDa (predicted molecular weight: 125 kDa).

Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below.

Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use a concentration of 5 µg/ml.
WB
1/1000. Detects a band of approximately 125-140 kDa (predicted molecular weight: 125 kDa).

Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below.

Target

  • Relevance

    Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
  • Database links

    • Entrez Gene: 1001 Human
    • Entrez Gene: 1002 Human
    • Entrez Gene: 1003 Human
    • Entrez Gene: 1004 Human
    • Entrez Gene: 1005 Human
    • Entrez Gene: 1006 Human
    • Entrez Gene: 1008 Human
    • Entrez Gene: 1009 Human
    • Entrez Gene: 1012 Human
    • Entrez Gene: 1014 Human
    • Entrez Gene: 1015 Human
    • Entrez Gene: 999 Human
    • Omim: 114020 Human
    • Omim: 114021 Human
    • Omim: 192090 Human
    • Omim: 601120 Human
    • Omim: 603006 Human
    • SwissProt: P12830 Human
    • SwissProt: P19022 Human
    • SwissProt: P22223 Human
    • SwissProt: P33151 Human
    • SwissProt: P55283 Human
    • SwissProt: P39038 Mouse
    see all
  • Alternative names

    • Cadherin antibody
    • CDH3 antibody
    • CDHP antibody
    • 7B4 antigen antibody
    • Cadherin-1 antibody
    • Cadherin-2 antibody
    • Cadherin-3 antibody
    • Cadherin-4 antibody
    • Cadherin-5 antibody
    • CAM 120/80 antibody
    • CD144 antibody
    • CD324 antibody
    • CD325 antibody
    • Cdh4 antibody
    • CDH5 antibody
    • CDHE antibody
    • CDHN antibody
    • E-cadherin antibody
    • Epithelial cadherin antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • P-cadherin antibody
    • Placental cadherin antibody
    • UVO antibody
    • Uvomorulin antibody
    • Vascular endothelial cadherin antibody
    see all

Images

  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Lane 1 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% BSA)
    Lane 2 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% Milk)

    All lanes : Heart (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 125 kDa


    Exposure time: 30 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

    ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

  • Flow Cytometry - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Flow Cytometry - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Overlay histogram showing HEK293 cells stained with ab22744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22744, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    All lanes : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate (blocked with 5% Milk)
    Lane 2 : Heart (Mouse) Tissue Lysate (blocked with 5% Milk)
    Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 5% Milk)
    Lane 4 : Heart (Rat) Tissue Lysate (blocked with 3% Milk)
    Lane 5 : Heart (Mouse) Tissue Lysate (blocked with 3% Milk)
    Lane 6 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 3% Milk)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 125 kDa
    Observed band size: 125 kDa
    Additional bands at: 25 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)Image courtesy of an anonymous Abreview.
    ab22744 staining pan Cadherin in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol, permeabilised in 0.5% (w/v) saponin and then blocked using 10% serum for 2 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 2 hours at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. Counterstained with DAPI (blue).

    See Abreview

  • Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Western blot - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)This image is courtesy of an Anonymous abreview.
    Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1/500 dilution + Mouse cultured cortical neurons at 20 µg

    Secondary
    HRP-conjugated Goat Anti-Mouse IgG (H+L) polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 125 kDa


    Exposure time: 1 minute


    Blocking performed with 5% milk for 1 hour.
    Primary diluted with PBS + 0.5% Tween20 and incubated for 12 hours at 4°C
    Performed under denaturing conditions.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody [mAbcam22744] (ab22744)

    ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (16)

    Publishing research using ab22744? Please let us know so that we can cite the reference in this datasheet.

    ab22744 has been referenced in 16 publications.

    • Deng Q  et al. Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells. Int J Endocrinol 2018:4537214 (2018). PubMed: 29686703
    • Storman EM  et al. Physical Linkage of Estrogen Receptor a and Aromatase in Rat: Oligocrine and Endocrine Actions of CNS-Produced Estrogens. Endocrinology 159:2683-2697 (2018). PubMed: 29771302
    • Zemel BM  et al. Calcineurin Dysregulation Underlies Spinal Cord Injury-Induced K+ Channel Dysfunction in DRG Neurons. J Neurosci 37:8256-8272 (2017). PubMed: 28751455
    • Deng Q  et al. Non-Genomic Action of Androgens is Mediated by Rapid Phosphorylation and Regulation of Androgen Receptor Trafficking. Cell Physiol Biochem 43:223-236 (2017). PubMed: 28854419
    • Xia Z  et al. Zebrafish slc30a10 deficiency revealed a novel compensatory mechanism of Atp2c1 in maintaining manganese homeostasis. PLoS Genet 13:e1006892 (2017). ICC/IF ; Zebrafish . PubMed: 28692648
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (J774A.1 macrophages)
    Permeabilization
    Yes - 0.05% saponin
    Specification
    J774A.1 macrophages
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 15 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mixed glia prepared from mouse brain)
    Specification
    mixed glia prepared from mouse brain
    Fixative
    Methanol
    Permeabilization
    Yes - 0,5%(w/v) saponin
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 26 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (293 human embryonic kidney)
    Specification
    293 human embryonic kidney
    Fixative
    4%(w/v) Paraformaldehyde + 4%(w/v) sucrose
    Permeabilization
    Yes - 0,5%(w/v) saponin
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cadherin antibody [mAbcam22744] - Plasma Membrane Marker

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (keratinocyte)
    Specification
    keratinocyte
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 12 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.