Overview

  • Product name

    Anti-pan Cadherin antibody [mAbcam22744] (HRP)
    See all pan Cadherin primary antibodies
  • Description

    Mouse monoclonal [mAbcam22744] to pan Cadherin (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human pan Cadherin aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: Rat, mouse and human heart tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab197725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).

Target

Images

  • All lanes : Anti-pan Cadherin antibody [mAbcam22744] (HRP) (ab197725) at 1/5000 dilution

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 125 kDa
    Observed band size: 125 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197725 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab197725 has not yet been referenced specifically in any publications.

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