Overview

  • Product name

    pan Caspase (active) Red Staining Kit
    See all Caspase kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Assay time

    2h 00m
  • Product overview

    Abcam's pan Caspase (active) Red Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes the caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent in situ marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

    Other caspase and apoptosis assays

    Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Red-VAD-FMK 1 x 100µl
    Wash Buffer 2 x 100ml
    Z-VAD-FMK 1 x 10µl
  • Research areas

  • Relevance

    Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • Cellular localization

    Cytoplasmic

Protocols

References

ab65616 has not yet been referenced specifically in any publications.

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