Anti-pan Cytokeratin antibody [AE-1] - BSA and Azide free (ab213135)
Key features and details
- Mouse monoclonal [AE-1] to pan Cytokeratin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Monkey, Turtle
- Isotype: IgG1
Overview
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Product name
Anti-pan Cytokeratin antibody [AE-1] - BSA and Azide free
See all pan Cytokeratin primary antibodies -
Description
Mouse monoclonal [AE-1] to pan Cytokeratin - BSA and Azide free -
Host species
Mouse -
Specificity
ab187381 recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily.
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Monkey, Turtle -
Immunogen
Recombinant full length protein. P13645 (CK10); P02533 (CK14); P19012 (CK15); P08789 (CK16); P08727 (CK19)
Database link: P13645 -
Positive control
- Skin, Squamous cell carcinoma (SCC)
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General notes
ab213135 is a carrier free version of ab187381. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AE-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab213135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody (ab213135)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab213135 has not yet been referenced specifically in any publications.
