Key features and details
- Mouse monoclonal [AE1/AE3] to pan Cytokeratin
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Human, Pig, Macaque monkey
- Isotype: IgG1
Product nameAnti-pan Cytokeratin antibody [AE1/AE3]
See all pan Cytokeratin primary antibodies
DescriptionMouse monoclonal [AE1/AE3] to pan Cytokeratin
SpecificityAb27988 recognises Pan cytokeratin.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Human, Pig, Macaque monkey
Full length protein: Human epidermal keratins.
- Human skin, oesophagus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab27988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product requires protein digestion pretreatment (e.g. trypsin or pronase), or antigen retrieval using heat treatment prior to staining of paraffin sections.
|IHC-Fr||1/10 - 1/40.|
RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
- pan ck antibody
- pan-ck antibody
- panck antibody
Ab27988 at 1/10 dilution staining formalin fixed paraffin embedded human oesophagus by Immunohistochemisty.
ab27988 staining Cytokeratin in Mouse tissue sections (Human tumor xenograft (Breast carcinoma)) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with MOM kit from Vector Laboratories for 1 hour at 15°C; antigen retrieval was by enzymatic in proteinase K. Samples were incubated with primary antibody (1/20) for 30 minutes at 15°C. A Biotin-conjugated Goat anti-mouse polyclonal (1/10) was used as the secondary antibody.
ab27988 has been referenced in 37 publications.
- Augustine T et al. Sensitization of colorectal cancer to irinotecan therapy by PARP inhibitor rucaparib. Invest New Drugs N/A:N/A (2019). PubMed: 30612311
- Yee CH et al. Maintenance of the bladder cancer precursor urothelial hyperplasia requires FOXA1 and persistent expression of oncogenic HRAS. Sci Rep 9:270 (2019). PubMed: 30670749
- Baumann B et al. Association of High miR-182 Levels with Low-Risk Prostate Cancer. Am J Pathol 189:911-923 (2019). PubMed: 30703341
- Shimura S et al. Selective Neck Dissection and Survival in Pathologically Node-Positive Oral Squamous Cell Carcinoma. Cancers (Basel) 11:N/A (2019). PubMed: 30823588
- Mano Y et al. Bone morphogenetic protein 4 provides cancer-supportive phenotypes to liver fibroblasts in patients with hepatocellular carcinoma. J Gastroenterol N/A:N/A (2019). PubMed: 30941514