Overview

  • Product name
    Anti-pan Cytokeratin antibody [AE1/AE3]
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [AE1/AE3] to pan Cytokeratin
  • Host species
    Mouse
  • Specificity
    Ab27988 recognises Pan cytokeratin.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig, Macaque monkey
  • Immunogen

    Full length protein: Human epidermal keratins.

  • Positive control
    • Human skin, oesophagus.

Properties

Applications

Our Abpromise guarantee covers the use of ab27988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This product requires protein digestion pretreatment (e.g. trypsin or pronase), or antigen retrieval using heat treatment prior to staining of paraffin sections.

IHC-Fr 1/10 - 1/40.

Target

  • Relevance
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization
    Cytoplasmic
  • Database links

Images

  • Ab27988 at 1/10 dilution staining formalin fixed paraffin embedded oesophagus by Immunohistochemisty.
  • ab27988 staining Cytokeratin in Mouse tissue sections (Human tumor xenograft (Breast carcinoma)) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with MOM kit from Vector Laboratories for 1 hour at 15°C; antigen retrieval was by enzymatic in proteinase K. Samples were incubated with primary antibody (1/20) for 30 minutes at 15°C. A Biotin-conjugated Goat anti-mouse polyclonal (1/10) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Williams J  et al. Epithelial and stromal circadian clocks are inversely regulated by their mechano-matrix environment. J Cell Sci 131:N/A (2018). Read more (PubMed: 29361531) »
  • Jemail L  et al. Pivotal roles of Kupffer cells in the progression and regression of DDC-induced chronic cholangiopathy. Sci Rep 8:6415 (2018). Read more (PubMed: 29686325) »
See all 15 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Nov 09 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Nov 08 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
Tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Nov 08 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Nov 08 2018

Question
Answer

The concentration of this lot of ab27988 Anti-pan Cytokeratin antibody [AE1/AE3] is 80 µg/ml.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Human tumor xenograft (Breast carcinoma))
Specification
Human tumor xenograft (Breast carcinoma)
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K at 37C for 15min
Permeabilization
No
Blocking step
Used MOM Kit from Vector Laboratories as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 15°C

Abcam user community

Verified customer

Submitted Jul 31 2012

Answer

Thank you for contacting Abcam regarding ab27988.


The antigen retrieval buffer we would recommend for this antibody is for Tris/EDTA.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Answer

Thank you for contacting us with your question.

I have looked throughourrecords, and this antibody has actually been tested with mouse samples and it does cross-react with this species. So, it will not be suitable for your assay, and unfortunately we do not have any other pan-cytokeratin antibodies that are known to not react with mouse.I apologize that this information was not previously on the datasheet of ab27988, and I'll update it accordingly.

Please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help.

Read More

Answer

Thank you for your inquiry.

For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig cytokeratin 8 sequence. We generally say anything with >85% homology would be expected to cross-react, so this is right on the edge. However, we do have two anti-cytokeratin 8 antibodies that we know do work in pig as ab2531 and ab2530.

https://www.abcam.com/Cytokeratin-8-antibody-C-51-ab2531.html

https://www.abcam.com/Cytokeratin-8-antibody-C-43-ab2530.html

For ab27988, this antibody has been validated in pig and we therefore guarantee it will work for pig in IHC-P, IHC-Fr in accordance with our Abpromise.

For ab7504, the immunogen was human breast carcinoma cell line, MCF-7. Pig and human EpCAM is 83% homologous, so this is a little low. We have an anti-EpCAM that is predicted (>90% homology) to cross-react with pig, and that is ab71916. However, this hasn't been validated and therefore not guaranteed in pig.

https://www.abcam.com/EpCAM-antibody-ab71916.html

I have to check with the lab on ab32362 and ab124687 since I don't have the immunogens readily available to BLAST.

For ab28028, full length native protein (purified): Vimentin isolated from bovine eye lens was used as an immunogen. Pig vimentin and bovine vimentin are 97% homologous, so there is a good chance this will cross-react with pig. However this hasn't been tested and therefore isn't validated in pig. We do have a few vimentin antibodies that work in pig though,like ab20346 and ab8069.

https://www.abcam.com/Vimentin-antibody-VI-10-ab20346.html

https://www.abcam.com/Vimentin-antibody-V9-ab8069.html

For ab11333, cultured human thymic fibroblasts was used as an immunogen. We don't have a particular protein sequence to look up for this one, so unfortunately we don't know how/if it will work with pig.

I hope this information helps. I'll be in touch again once I hear about ab32362 and ab124687.

Read More

Answer

Thank you for your enquiry and your interest in our products.

I can confirm that neither of these antibodies has been tested for cross-reactivity with mouse. If you wish to test one of them in mouse system, I could offer you a testing discount.

The Terms and Conditions of the testing discount can be found at: https://www.abcam.com/collaborationdiscount.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up