Anti-pan Cytokeratin antibody [AE1/AE3] (ab27988)


  • Product name
    Anti-pan Cytokeratin antibody [AE1/AE3]
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [AE1/AE3] to pan Cytokeratin
  • Host species
  • Specificity
    Ab27988 recognises Pan cytokeratin.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig, Macaque monkey
  • Immunogen

    Full length protein: Human epidermal keratins.

  • Positive control
    • Human skin, oesophagus.



Our Abpromise guarantee covers the use of ab27988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This product requires protein digestion pretreatment (e.g. trypsin or pronase), or antigen retrieval using heat treatment prior to staining of paraffin sections.

IHC-Fr 1/10 - 1/40.


  • Relevance
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization
  • Database links


  • Ab27988 at 1/10 dilution staining formalin fixed paraffin embedded oesophagus by Immunohistochemisty.
  • ab27988 staining Cytokeratin in Mouse tissue sections (Human tumor xenograft (Breast carcinoma)) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with MOM kit from Vector Laboratories for 1 hour at 15°C; antigen retrieval was by enzymatic in proteinase K. Samples were incubated with primary antibody (1/20) for 30 minutes at 15°C. A Biotin-conjugated Goat anti-mouse polyclonal (1/10) was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Williams J  et al. Epithelial and stromal circadian clocks are inversely regulated by their mechano-matrix environment. J Cell Sci 131:N/A (2018). Read more (PubMed: 29361531) »
  • Jemail L  et al. Pivotal roles of Kupffer cells in the progression and regression of DDC-induced chronic cholangiopathy. Sci Rep 8:6415 (2018). Read more (PubMed: 29686325) »

See all 15 Publications for this product

Customer reviews and Q&As

The concentration of this lot of ab27988 Anti-pan Cytokeratin antibody [AE1/AE3] is 80 µg/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Human tumor xenograft (Breast carcinoma))
Human tumor xenograft (Breast carcinoma)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K at 37C for 15min
Blocking step
Used MOM Kit from Vector Laboratories as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 15°C

Abcam user community

Verified customer

Submitted Jul 31 2012

Thank you for contacting Abcam regarding ab27988.

The antigen retrieval buffer we would recommend for this antibody is for Tris/EDTA.

I hope this information is helpful. Please do not hesitate to contact me if you have any a...

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Thank you for contacting us with your question.

I have looked throughourrecords, and this antibody has actually been tested with mouse samples and it does cross-react with this species. So, it will not be suitable for your assay, and unfortun...

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Thank you for your inquiry.

For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

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Thank you for your enquiry and your interest in our products.

I can confirm that neither of these antibodies has been tested for cross-reactivity with mouse. If you wish to test one of them in mouse system, I could offer you a testing discoun...

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Thanks for your enquiry.

For this antibody, the exact amount of specific antibody present in certain antibody preparations is not measured. The approximate concentration range for this product is 25 - 200 ug/ml.

I hope this is helpf...

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Thank you for your enquiry.

This antibody has been succesfully tested to react with formalin fixed sections. As far as we know ab27988 has not yet been tested for reactivity to rat so we have no data to suggest if it will or will not detect r...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (skin, epidermis)
skin, epidermis
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8.0
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C

Mr. Erwin Kump

Verified customer

Submitted Aug 19 2008


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