Overview

  • Product name

    Anti-pan Cytokeratin antibody [AE1/AE3] - BSA and Azide free
    See all pan Cytokeratin primary antibodies
  • Description

    Mouse monoclonal [AE1/AE3] to pan Cytokeratin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, IMC™more details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Monkey
  • Immunogen

    Full length native protein (purified) corresponding to Human pan Cytokeratin.

  • Positive control

    • Skin. Lung carcinoma, human tonsil tissue IF: HepG2 cell line IMC: Human lung cancer tissue
  • General notes

    This product was changed from ascites to tissue culture supernatant on 12th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab80826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Boiling tissue sections in 1mM EDTA (pH 8.0), for 10-20min followed by cooling at RT for 20min is required.

IMC™ Use at an assay dependent concentration.

Target

  • Relevance

    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • pan ck antibody
    • pan-ck antibody
    • panck antibody

Images

  • Imaging Mass Cytometry™ (IMC™) image of human lung cancer tissue stained with Anti-pan Cytokeratin antibody [AE1/AE3]. ab80826 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

    Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue with ab80826 at 1/400 dilution. Anti-Mouse HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using citrate based pH 6.0 buffer.

    This image was generated using the ascites version of the product.

  • ICC/IF image of ab80826 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80826, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing A431 cells stained with ab80826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human skin tissue using 1/50 ab80826, a peroxidase-conjugated secondary antibody and an AEC chromogen. Note cytoplasmic staining of epithelial cells.

    This image was generated using the ascites version of the product.

  • Immunohistochemical analysis of rat bladder tissue, labeling pan Cytokeratin with ab80826. Samples were formaldehyde fixed, heat mediated antigen retrieval was perfmormed with 10mM citrate buffer, and blocking was with 10% serum for 2 hours at 23°C. Incubation with ab80826 (diluted 1/1000) was for 17 hours at 4°C.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:

  • Melissaridou S  et al. The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer. Cancer Cell Int 19:16 (2019). Read more (PubMed: 30651721) »
  • Skurikhin EG  et al. Endothelial Progenitor Cells as Pathogenetic and Diagnostic Factors, and Potential Targets for GLP-1 in Combination with Metabolic Syndrome and Chronic Obstructive Pulmonary Disease. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30836679) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Sample
Rat Tissue sections (bladder)
Specification
bladder
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 26 2014

Answer

Merci de nous avoir contactés.

L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration en anticorps ne peut donc pas être déterminée précisement mais elle est estimée entre 1 et 3 mg/ml.
Malheureusement le laboratoire qui produit l'anticorps ab961 (Anti-pan Cytokeratin antibody [AE1+AE3]) ne désire pas communiquer la concentration de ce produit. Nous sommes désolés de ne pas pouvoir vous fournir cette information, sachez cependant que nous avons au catalogue une référence équivalente, non-prédiluée, ab80826, à 1 mg/ml, https://www.abcam.com/ab80826.




J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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