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Human epidermal keratin
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab7753 as a replacement.
Our Abpromise guarantee covers the use of ab80826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boiling tissue sections in 1mM EDTA (pH 8.0), for 10-20min followed by cooling at RT for 20min is required.|
Immunohistochemical analysis of rat bladder tissue, labeling pan Cytokeratin with ab80826. Samples were formaldehyde fixed, heat mediated antigen retrieval was perfmormed with 10mM citrate buffer, and blocking was with 10% serum for 2 hours at 23°C. Incubation with ab80826 (diluted 1/1000) was for 17 hours at 4°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"