Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)

Reviews (2)Q&A (6)References (19)

Overview

  • Product name
    Anti-pan Cytokeratin antibody [AE1+AE3], prediluted
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted
  • Host species
    Mouse
  • Specificity
    This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish
  • Immunogen

    Human epidermal cytokeratins

  • Positive control
    • Skin or adenocarcinoma.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19852942
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization
    Cytoplasmic
  • Database links

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)Image from Liu J et al, Biochem Biophys Res Commun. 2009 Dec 18;390(3):931-6. Epub 2009 Oct 21, Fig 1. DOI 10.1016/j.bbrc.2009.10.080
      ab961 at a 1/100 dilution staining pan Cytokeratin in human urothelium-like cells in culture by Immunocytochemistry/ Immunofluorescence. Counterstained with DAPI.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)Image from Jung IH et al., PLoS One. 2011;6(12):e27941. Epub 2011 Dec 2. Fig 7.; doi:10.1371/journal.pone.0027941 December 2, 2011, PLoS ONE 6(12): e27941.
      Immunohistochemical analysis of zebrafish pancreatic tissue, staining pan Cytokeratin with ab961, at 1/500 dilution.

    References

    This product has been referenced in:
    • Ramírez R & Marshall SH Identification and isolation of infective filamentous particles in Infectious Salmon Anemia Virus (ISAV). Microb Pathog 117:219-224 (2018). Read more (PubMed: 29454823) »
    • Liu DN  et al. Superior mesenteric artery margin in pancreaticoduodenectomy for pancreatic adenocarcinoma. Oncotarget 8:7766-7776 (2017). Read more (PubMed: 27999192) »
    See all 19 Publications for this product

    Publishing research using ab961? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-8 of 8 Abreviews or Q&A

    Question

    Inquiry: Dear Sir I WOULD LIKE TO KNOW THAT WHETHER WE SHOULD DILUTE THIS PREDILUTED ANTIBODY FOR IMMUNOCYTOCHEMISTRY OR OTHER APPLICATIONS IN DATA SHEET IT IS WRITTEN AS USE ASSAY DEPENDENT DILUTION.THEN WHY DOES PRE DILUTED MEANS EXPECTING YOUR REPLY

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    Answer

    Thank you for contacting us.

    I can confirm that the Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961) can be used for applications without further dilutions.

    However, every experiment is different and may need optimisation by the user. Some customers seem to get excellent results by diluting this prediluted antibody further. We would recommend to optimise the experiment by trying the undiluted antibody and if possible dilutions found in the literature.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Question

    Bonjour,
    j'aimerai avoir des renseignements concernant les anticorps de
    référence:
    - ab82612
    - ab28028
    - ab961
    Je n'ai pas trouvé la concentration de ces anticorps sur les fiches
    techniques. 2 de ces 3 anticorps sont des anticorps prédilués, mais
    serait-il possible de connaître dans quelle gamme de concentration ils
    sont fournis?
    Je vous remercie
    Bien cordialement

    Read More
    Answer

    Merci de nous avoir contactés.

    L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
    L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration en anticorps ne peut donc pas être déterminée précisement mais elle est estimée entre 1 et 3 mg/ml.
    Malheureusement le laboratoire qui produit l'anticorps ab961 (Anti-pan Cytokeratin antibody [AE1+AE3]) ne désire pas communiquer la concentration de ce produit. Nous sommes désolés de ne pas pouvoir vous fournir cette information, sachez cependant que nous avons au catalogue une référence équivalente, non-prédiluée, ab80826, à 1 mg/ml, https://www.abcam.com/ab80826.




    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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    Question

    To Whom It May Concern:

    I would like to ask you for anti-pan Cytokeratin antibody product: What is the different between anti-pan cytokeratin antibody(PCK-26) code # ab6401 and anti-pan cytokeratin antibody (PCK-26) code # ab11214. ?. I also would like to know more information for your product, anti-pan Cytokeratin antibody (AE1+AE3) prediluted code # ab961. Thank you.

    Read More
    Answer

    Thank you for contacting us.

    The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11214 is purified and conjugated to FITC.

    The mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted, ab961 is already diluted and ready to use for cell and tissue staining right out of the vial. You receive 6 ml of this pre-diluted antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [AE1+AE3], prediluted

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Dog-Kidney)
    Specification
    Dog-Kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rm°C
    Read More

    Jing Ma

    Verified customer

    Submitted Aug 30 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [AE1+AE3], prediluted

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor)
    Specification
    tumor
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 01 2009

    Question

    I wonder if this antibody stains normal respiratory epithelia in the rat as it specific for cytokearitins. If "yes", can you provide me the references? I will very appreciate your response.

    Read More
    Answer

    Thank you for your enquiry and patience. I was able to obtain the following information from the originators of these antibodies: For ab11213, "this product should recognise cytokeratins 1, 4, 5, 6, 8,10, 13, 18 and 19. Based on literature, respiratory epithelia expressses cytokeratins 5, 6, 7, and 8. Thus I would believe this antibody would prove suitable but I would not have any references available specific for the use of this antibody for rat respiratory epithelia." For ab8794, "we have tested this antibody on skin, colon, pancreas and several other epithelial tissues. We have not tested in respiratory epithelia in rat, although this antibody has broad species reactivity, reacts with mouse, rat bovine, others not tested." I haven't been able to obtain any additional information regarding ab961. If I do, I will pass it on to you. This antibody is a combination of 2 clones and recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52. Please contact us again if you have any additional questions.

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    Question

    What is the carrier protein? Is there a concentration available?

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    Answer

    Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

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    Question

    Will this antibody work on mouse kidney tissue?

    Read More
    Answer

    According to the datasheet, this antibody recognizes mouse protein. However, we do not have data on mouse kidney tissue particularly. Please have a look at the published references, you may find some more information.

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