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  1. Link

    pan-cytokeratin-antibody-ae1ae3-prediluted-ab961.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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Recombinant

Recombinant Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)

  • Datasheet
Reviews (2)Q&A (5)References (35)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse recombinant multiclonal [AE1+AE3] to pan Cytokeratin, prediluted
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish

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Overview

  • Product name

    Anti-pan Cytokeratin antibody [AE1+AE3], prediluted
    See all pan Cytokeratin primary antibodies
  • Description

    Mouse recombinant multiclonal [AE1+AE3] to pan Cytokeratin, prediluted
  • Host species

    Mouse
  • Specificity

    This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish
  • Immunogen

    Tissue, cells or virus corresponding to pan Cytokeratin. Human epidermal cytokeratins

  • Positive control

    • Skin or adenocarcinoma.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Proprietary Preservative
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Recombinant Multiclonal
  • Clone number

    AE1+AE3
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Unmasking reagent

    • Trypsin Antigen Retrieval Solution (ab970)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 19852942
Notes
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 19852942

Target

  • Relevance

    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 374454 Human
    • Entrez Gene: 51350 Human
    • SwissProt: Q01546 Human
    • SwissProt: Q7Z794 Human
    • Alternative names

      • pan ck antibody
      • pan-ck antibody
      • panck antibody

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (35)

    Publishing research using ab961? Please let us know so that we can cite the reference in this datasheet.

    ab961 has been referenced in 35 publications.

    • Jin YP  et al. Encapsulated three-dimensional bioprinted structure seeded with urothelial cells: a new construction technique for tissue-engineered urinary tract patch. Chin Med J (Engl) 133:424-434 (2020). PubMed: 31977553
    • Tan J  et al. An R-loop-initiated CSB-RAD52-POLD3 pathway suppresses ROS-induced telomeric DNA breaks. Nucleic Acids Res 48:1285-1300 (2020). PubMed: 31777915
    • Wan X  et al. Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis. Stem Cell Res Ther 11:535 (2020). PubMed: 33308306
    • Gong J  et al. Reprogramming of lipid metabolism in cancer-associated fibroblasts potentiates migration of colorectal cancer cells. Cell Death Dis 11:267 (2020). PubMed: 32327627
    • St-Jean G  et al. Lats1 and Lats2 are required for the maintenance of multipotency in the Müllerian duct mesenchyme. Development 146:N/A (2019). PubMed: 31575647
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [AE1+AE3], prediluted

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Dog-Kidney)
    Specification
    Dog-Kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rm°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Jing Ma

    Verified customer

    Submitted Aug 30 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [AE1+AE3], prediluted

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor)
    Specification
    tumor
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 01 2009

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    For licensing inquiries, please contact partnerships@abcam.com

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