Anti-pan Cytokeratin antibody [C-11] (ab7753)
- Datasheet
- References (32)
- Protocols
Overview
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Product nameAnti-pan Cytokeratin antibody [C-11]
See all pan Cytokeratin primary antibodies -
DescriptionMouse monoclonal [C-11] to pan Cytokeratin
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Host speciesMouse
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SpecificityCytokeratin peptides 4,5,6,8,10,13,18.
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, WB, IPmore details
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Species reactivityReacts with: Mouse, Rat, Goat, Cow, Human, Pig, Common marmoset, Bat
Predicted to work with: a wide range of other species, Mammals
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Immunogen
A keratin-enriched preparation from human epidermoid carcinoma cell line A431.
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Positive control
- ICC/IF: A549 and mouse hepatocytes (see reviews). WB: A431 cell lysate. Flow Cyt: HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity>95% by SDS-PAGE
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Purification notesPurified from TCS
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ClonalityMonoclonal
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Clone numberC-11
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab7753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250 - 1/500. Fix with 4% PFA and permeabilize with 0.5 % Triton (See Abreview). | |
| Flow Cyt | Use a concentration of 0.5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/1000. 1/500 - 1/1000 (See Abreview). | |
| IP | Use at an assay dependent concentration. |
Target
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RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/pan-Cytokeratin-Primary-antibodies-ab7753-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbsab7753 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab7753 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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![Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-262982-ab7753IHC.jpg)
Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbsab7753 staining pan Cytokeratin in Rat urinary bladder tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C. Samples were incubated with primary antibody (1/150 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-262980-ab7753ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)Image courtesy of an anonymous Abreviewab7753 staining pan Cytokeration in the Human NSCLC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in 0.3% Triton, 5% Goat Serum in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat polyclonal (1/500) was used as the secondary antibody.
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![Western blot - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/pan-Cytokeratin-Primary-antibodies-ab7753-1.jpg)
Western blot - Anti-pan Cytokeratin antibody [C-11] (ab7753)Anti-pan Cytokeratin antibody [C-11] (ab7753) + human A431 cell line on 10% gel.
This is a pan cytokeratin antibody and there are several cytokeratin bands between about 40 and 55 kDa. The identity of the particular cytokeratin that each band corresponds to has not been determined. -
![Flow Cytometry - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/pan-Cytokeratin-Primary-antibodies-ab7753-15.jpg)
Flow Cytometry - Anti-pan Cytokeratin antibody [C-11] (ab7753)Overlay histogram showing HeLa cells stained with ab7753 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7753, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/pan-Cytokeratin-Primary-antibodies-ab7753-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbsab7753 staining rat embryonic skin/organ sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab77539 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
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![Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/pan-Cytokeratin-Primary-antibodies-ab7753-19.jpg)
Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Beate Thodeab7753 staining pan Cytokeratin in Pig urether tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500) for 3 hours at 20°C. A Cy2®-conjugated Goat anti-mouse monoclonal (1/200) was used as the secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-262981-ab7753ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)ab7753 staining pan Cytokeration in Cow Airway Epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% serum for 15 minutes at 37°C. Samples were incubated with primary antibody (1/500 in PBS/1%BSA) for 1 hour at 37°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/50) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-262983-ab7753IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbsab7753 staining pan Cytokeratin in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/250 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-242672-ab7753iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an anonymous Abreviewab7753 staining pan Cytoketatin in mouse hepatocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 0.5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A Cy3®-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753_5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)ab7753 staining pan Cytokeratin in guinae pig breast carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Protocols
References
This product has been referenced in:
- Pellinen T et al. ITGB1-dependent upregulation of Caveolin-1 switches TGFß signalling from tumour-suppressive to oncogenic in prostate cancer. Sci Rep 8:2338 (2018). IHC . Read more (PubMed: 29402961) »
- Mertens C et al. Macrophage-derived lipocalin-2 transports iron in the tumor microenvironment. Oncoimmunology 7:e1408751 (2018). Read more (PubMed: 29399416) »
