Name: Anti-pan Cytokeratin antibody [C-11]
SKU: ab7753
Rating: 4 (23 reviews)

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Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [C-11] to pan Cytokeratin
Suitable for: IHC-P, mIHC, ICC/IF, IHC-Fr
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Carrier Free

Product name

Anti-pan Cytokeratin antibody [C-11]
See all pan Cytokeratin primary antibodies
Description

Mouse monoclonal [C-11] to pan Cytokeratin
Host species

Mouse
Specificity

Cytokeratin peptides 4,5,6,8,10,13,18.
Tested applications

Suitable for: IHC-P, mIHC, ICC/IF, IHC-Frmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Tissue, cells or virus corresponding to Human pan Cytokeratin. Mixture of purified keratins
Positive control
- IHC-Fr: Rat kidney, mouse large intestine and human skin. IHC-P: Rat, mouse and human skin. ICC/IF: HeLa and A431 cells. mIHC: Human tonsil tissue and Human breast cancer tissue.
General notes
This product has switched from a hybridoma to recombinant production method on 08^th March 2021
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS. Purity >95% by SDS-PAGE.
Clonality

Monoclonal
Clone number

C-11
Isotype

IgG1
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(10)	Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
mIHC		Use at an assay dependent concentration.
ICC/IF	(6)	Use a concentration of 1 µg/ml.
IHC-Fr	(3)	Use a concentration of 1 µg/ml.

Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
mIHC Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.

Relevance

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization

Cytoplasmic
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody

Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of ImmunoAtlas.

Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of Anti-PD-L1 (ab251611), Anti-Granzyme B (ab219803), Anti-PD1 (ab251613), Anti-pan Cytokeratin (ab264485), Anti-EpCAM (ab225894), Anti-CD8 alpha (ab251596) and Anti-FOXP3 (ab96048).

The immunostaining was performed on a Leica Biosystems BOND^® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences^®).

The section was incubated in six rounds of staining; sequentially for ab251611 (1/750 dilution), ab219803 (1/250 dilution), ab251613 (1/750 dilution), ab264485 (0.5 µg/ml), ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and ab96048 (10 µg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND^® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences^®).

This data is courtesy of ImmunoAtlas and it can be found here.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

IHC image of pan cytokeratin staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6,epitope retrieval solution 1) for 20mins. The section was then incubated with ab7753, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions,primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank,supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)

ab7753 staining pan Cytokeratin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min),permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7753 at 1µg/ml and ab6046,Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117,Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080,Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS,Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Image was acquired with a high-content analyser (Operetta CLS,Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of ImmunoAtlas.

Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of Anti-PD-L1 (ab251611), Anti-Granzyme B (ab219803), Anti-PD1 (ab251613), Anti-pan Cytokeratin (ab264485), Anti-EpCAM (ab225894), Anti-CD8 alpha (ab251596) and Anti-FOXP3 (ab96048).

The immunostaining was performed on a Leica Biosystems BOND^® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences^®).

The section was incubated in six rounds of staining; sequentially for ab251611 (1/750 dilution), ab219803 (1/250 dilution), ab251613 (1/750 dilution), ab264485 (0.5 µg/ml), ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and ab96048 (10 µg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND^® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences^®).

This data is courtesy of ImmunoAtlas and it can be found here.
Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of ImmunoAtlas.

Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of Anti-PD-L1 (ab251611), Anti-Granzyme B (ab219803), Anti-PD1 (ab251613), Anti-pan Cytokeratin (ab264485), Anti-EpCAM (ab225894), Anti-CD8 alpha (ab251596) and Anti-FOXP3 (ab96048).

The immunostaining was performed on a Leica Biosystems BOND^® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences^®).

The section was incubated in six rounds of staining; sequentially for ab251611 (1/750 dilution), ab219803 (1/250 dilution), ab251613 (1/750 dilution), ab264485 (0.5 µg/ml), ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and ab96048 (10 µg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND^® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences^®).

This data is courtesy of ImmunoAtlas and it can be found here.
Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

IHC image of pan cytokeratin staining in a section of frozen normal mouse large intestine performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab7753, 1µg/ml, for 15 mins at room temperature. A goat anti-mouse IgG1 bridging antibody, ab125913, was added for 8 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of ImmunoAtlas.

Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of Anti-PD-L1 (ab251611), Anti-Granzyme B (ab219803), Anti-PD1 (ab251613), Anti-pan Cytokeratin (ab264485), Anti-EpCAM (ab225894), Anti-CD8 alpha (ab251596) and Anti-FOXP3 (ab96048).

The immunostaining was performed on a Leica Biosystems BOND^® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences^®).

The section was incubated in six rounds of staining; sequentially for ab251611 (1/750 dilution), ab219803 (1/250 dilution), ab251613 (1/750 dilution), ab264485 (0.5 µg/ml), ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and ab96048 (10 µg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND^® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences^®).

This data is courtesy of ImmunoAtlas and it can be found here.
Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of ImmunoAtlas.

Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of Anti-PD-L1 (ab251611), Anti-Granzyme B (ab219803), Anti-PD1 (ab251613), Anti-pan Cytokeratin (ab264485), Anti-EpCAM (ab225894), Anti-CD8 alpha (ab251596) and Anti-FOXP3 (ab96048).

The immunostaining was performed on a Leica Biosystems BOND^® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences^®).

The section was incubated in six rounds of staining; sequentially for ab251611 (1/750 dilution), ab219803 (1/250 dilution), ab251613 (1/750 dilution), ab264485 (0.5 µg/ml), ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and ab96048 (10 µg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND^® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences^®).

This data is courtesy of ImmunoAtlas and it can be found here.
Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

IHC image of pan cytokeratin staining in a section of frozen normal rat kidney performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab7753,1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

IHC image of pan cytokeratin staining in a section of frozen normal human skin performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab7753, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

IHC image of pan cytokeratin staining in a section of formalin-fixed paraffin-embedded normal rat skin performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6,epitope retrieval solution 1) for 20mins. The section was then incubated with ab7753, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions,primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

IHC image of pan cytokeratin staining in a section of formalin-fixed paraffin-embedded normal mouse skin performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6,epitope retrieval solution 1) for 20mins. The section was then incubated with ab7753, 1µg/ml, for 15 mins at room temperature. A goat anti-mouse IgG1 bridging antibody, ab125913, was added for 8 mins at room temperature and detected using an HRP conjµgated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions,primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] (ab7753)

ab7753 staining pan Cytokeratin in A431 cells. The cells were fixed with 4% paraformaldehyde (10 min),permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7753 at 1µg/ml and ab6046,Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117,Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080,Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a high-content analyser (Operetta CLS,Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Image was acquired with a high-content analyser (Operetta CLS,Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)

This image was generated from the hybridoma version of the product.

Fluorescence multiplex immunohistochemical analysis of normal human tonsil tissue (formalin-fixed paraffin-embedded section).

Merged staining of anti-PD1 (ab237728; orange; Opal™520), anti-PDL1 (ab237726; green; Opal™540), anti-CD68 (ab192847; yellow; Opal™570), anti-CD3 (ab16669; red; Opal™620), anti-Ki67 (ab16667; light blue; Opal™650) and anti-PanCK (ab7753; grey; Opal™690).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT, Akoya Biosciences®).

The section was incubated in six rounds of staining; in the order of ab237728 (1/500 dilution), ab237726 (1/500 dilution), ab192847 (1/300 dilution), ab16669 (1/300 dilution), ab16667 (1/200 dilution) and ab7753 (1/200 dilution); each using a separate fluorescent tyramide signal amplification system.

Sodium citrate antigen retrieval (Leica ER1, pH6.0, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

DAPI (dark blue) was used as a nuclear counter stain.

Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra Polaris.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbs

This image was generated from the hybridoma version of the product.

ab7753 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab7753 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbs

This image was generated from the hybridoma version of the product.

ab7753 staining rat embryonic skin/organ sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab77539 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
See Abreview
Anti-pan Cytokeratin antibody [C-11] (ab7753)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Publishing research using ab7753? Please let us know so that we can cite the reference in this datasheet.

ab7753 has been referenced in 88 publications.

Fu X et al. Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis through the PI3K Pathway : Osteopontin Regulates Endometrial Cell Migration in Endometriosis. Reprod Sci 28:435-446 (2021). PubMed: 32909189
Li L et al. Laminin ?2-mediating T cell exclusion attenuates response to anti-PD-1 therapy. Sci Adv 7:N/A (2021). PubMed: 33536206
Rappl P et al. Inhibition of mPGES-1 attenuates efficient resolution of acute inflammation by enhancing CX3CL1 expression. Cell Death Dis 12:135 (2021). PubMed: 33542207
Kang SH et al. Cancer-Associated Fibroblast Subgroups Showing Differential Promoting Effect on HNSCC Progression. Cancers (Basel) 13:N/A (2021). PubMed: 33562096
Liu C et al. The value of circulating tumor cells with positive centromere probe 8 in the diagnosis of small pulmonary nodules. Transl Oncol 14:101052 (2021). PubMed: 33667891

View all Publications for this product

Customer reviews and Q&As

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1-10 of 29 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Pig Tissue sections (Skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH9

Permeabilization

Specification

Skin

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 09 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rabbit Tissue sections (Rabbit cornea. Limbal area.)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)

Permeabilization

Yes - PBT (0,1% triton-X-100 in PBS)

Specification

Rabbit cornea. Limbal area.

Blocking step

5%BSA and 10%FBS in PBS + 0,1% Triton as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Noelia Andollo

Verified customer

Submitted Jun 19 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (kidney, proximal tubule)

Permeabilization

Yes - 1% triton

Specification

kidney, proximal tubule

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Kara Shumansky

Verified customer

Submitted Mar 04 2019

Application

IHC - Wholemount

Sample

Mouse Tissue (Mammary adenocarcinoma)

Specification

Mammary adenocarcinoma

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Francesco Elia Marino

Verified customer

Submitted Sep 26 2018

Application

Western blot

Sample

Human Cell lysate - other (A549)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

10 µg

Specification

A549

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 06 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (Human primary airway epithelial and fibroblast cel)

Gel Running Conditions

Reduced Denaturing (12% gel)

Loading amount

10 µg

Specification

Human primary airway epithelial and fibroblast cel

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Aaron Gardner

Verified customer

Submitted Oct 24 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Primary pulmonary epithelial cells cultured at air)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate

Permeabilization

Specification

Primary pulmonary epithelial cells cultured at air

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Aaron Gardner

Verified customer

Submitted Oct 13 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HuCCA-1 (cholangiocarcinoma))

Permeabilization

Yes - 0.5% Tween-20 in PBS

Specification

HuCCA-1 (cholangiocarcinoma)

Blocking step

Blocking Buffer (ab126587) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

95% ethanol+acetic acid

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sittisak .

Verified customer

Submitted Aug 11 2016

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 23°C

Sample

Mouse Cell (hepatocytes)

Specification

hepatocytes

Permeabilization

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 20 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Sample

Pig Tissue sections (Small intestine)

Specification

Small intestine

Permeabilization

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Nov 20 2014

1-10 of 29 Abreviews or Q&A

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References (88)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [C-11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [C-11]

Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cytokeratin antibody [C-11]

IHC - Wholemount abreview for Anti-pan Cytokeratin antibody [C-11]

Western blot abreview for Anti-pan Cytokeratin antibody [C-11]

Western blot abreview for Anti-pan Cytokeratin antibody [C-11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [C-11]

Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cytokeratin antibody [C-11]

Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cytokeratin antibody [C-11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-pan Cytokeratin antibody [C-11]