Anti-pan Cytokeratin antibody [C-11] (ab7753)
Key features and details
- Mouse monoclonal [C-11] to pan Cytokeratin
- Suitable for: IHC-P, Flow Cyt, mIHC, WB
- Reacts with: Rat, Goat, Human, Common marmoset
- Isotype: IgG1
Overview
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Product name
Anti-pan Cytokeratin antibody [C-11]
See all pan Cytokeratin primary antibodies -
Description
Mouse monoclonal [C-11] to pan Cytokeratin -
Host species
Mouse -
Specificity
Cytokeratin peptides 4,5,6,8,10,13,18. -
Tested applications
Suitable for: IHC-P, Flow Cyt, mIHC, WBmore details -
Species reactivity
Reacts with: Rat, Goat, Human, Common marmoset
Predicted to work with: a wide range of other species, Mammals
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Immunogen
A keratin-enriched preparation from human epidermoid carcinoma cell line A431.
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Positive control
- ICC/IF: A549 and mouse hepatocytes (see reviews). WB: A431 cell lysate. Flow Cyt: HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
C-11 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab7753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 0.5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| mIHC | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. 1/500 - 1/1000 (See Abreview). |
Target
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Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Images
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![Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-383706-ab7753pdl1pd1multiplexihcifantibodypanelhumantonsilimmunohistochemistry.jpg)
Multiplex immunohistochemistry - Anti-pan Cytokeratin antibody [C-11] (ab7753)Fluorescence multiplex immunohistochemical analysis of normal human tonsil tissue (formalin-fixed paraffin-embedded section).
Merged staining of anti-PD1 (ab237728; orange; Opal™520), anti-PDL1 (ab237726; green; Opal™540), anti-CD68 (ab192847; yellow; Opal™570), anti-CD3 (ab16669; red; Opal™620), anti-Ki67 (ab16667; light blue; Opal™650) and anti-PanCK (ab7753; grey; Opal™690).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; in the order of ab237728 (1/500 dilution), ab237726 (1/500 dilution), ab192847 (1/300 dilution), ab16669 (1/300 dilution), ab16667 (1/200 dilution) and ab7753 (1/200 dilution); each using a separate fluorescent tyramide signal amplification system.
Sodium citrate antigen retrieval (Leica ER1, pH6.0, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.
DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra Polaris.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-13044-anti-pan-cytokeratin-antibody-c-11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbsab7753 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab7753 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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![Western blot - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-13041-anti-pan-cytokeratin-antibody-c-11-western-blot.jpg)
Western blot - Anti-pan Cytokeratin antibody [C-11] (ab7753)Anti-pan Cytokeratin antibody [C-11] (ab7753) + human A431 cell line on 10% gel.
This is a pan cytokeratin antibody and there are several cytokeratin bands between about 40 and 55 kDa. The identity of the particular cytokeratin that each band corresponds to has not been determined. -
![Flow Cytometry - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-13039-anti-pan-cytokeratin-antibody-c-11-flow-cytometry.jpg)
Flow Cytometry - Anti-pan Cytokeratin antibody [C-11] (ab7753)Overlay histogram showing HeLa cells stained with ab7753 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7753, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-13040-anti-pan-cytokeratin-antibody-c-11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbsab7753 staining rat embryonic skin/organ sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab77539 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-262983-anti-pan-cytokeratin-antibody-c-11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)This image is courtesy of an Abreview submitted by Carl Hobbsab7753 staining pan Cytokeratin in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/250 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)](https://www.abcam.com/ps/products/7/ab7753/Images/ab7753-13042-anti-pan-cytokeratin-antibody-c-11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)ab7753 staining pan Cytokeratin in guinae pig breast carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Protocols
References (60)
ab7753 has been referenced in 60 publications.
- Denu RA et al. Centrosome Amplification in Cancer Disrupts Autophagy and Sensitizes to Autophagy Inhibition. Mol Cancer Res 18:33-45 (2020). PubMed: 31604847
- Nelson L et al. A living biobank of ovarian cancer ex vivo models reveals profound mitotic heterogeneity. Nat Commun 11:822 (2020). PubMed: 32054838
- Pan H et al. Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis. Stem Cells Int 2020:5142797 (2020). PubMed: 32104185
- Davaadelger B et al. BRCA1 mutation influences progesterone response in human benign mammary organoids. Breast Cancer Res 21:124 (2019). PubMed: 31771627
- Mo TT et al. Optimized Generation of Primary Human Epithelial Cells from Larynx and Hypopharynx: A Site-Specific Epithelial Model for Reflux Research. Cell Transplant 28:630-637 (2019). PubMed: 30917697
