Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) (ab52460)

Mouse monoclonal pan Cytokeratin antibody [C-11] conjugated to PE. Validated in Flow Cyt. Cited in 6 publication(s). Immunogen corresponding to tissue, cells or virus.

Overview

  • Product name
    Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [C-11] to pan Cytokeratin (Phycoerythrin)
  • Host species
    Mouse
  • Specificity
    ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with all species (recognized epitope conserved within all species)
  • Immunogen

    Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.097% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
  • Clonality
    Monoclonal
  • Clone number
    C-11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1-2µl for 106 cells.

(in a 100µl cell suspension).

 

 

 

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization
    Cytoplasmic

Images

  • Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.

    Recommended conc. 0.5 μg/ml

    Overlay with Isotype mouse IgG1 control.

References

This product has been referenced in:
  • Guo YX  et al. Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer. Oncotarget 9:7522-7533 (2018). Read more (PubMed: 29484129) »
  • S Lashkari B & Anumba DO Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4. PLoS One 12:e0173646 (2017). Read more (PubMed: 28296959) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (kidney, epithelial)
Permeabilization
Yes - Biolegend Intracellular Staining Permeabilization Wash Buffer
Gating Strategy
all live cells
Specification
kidney, epithelial
Preparation
Cell harvesting/tissue preparation method: primary cells passaged and trypisinzed from flask at p2
Sample buffer: Biolegend Cell Staining Buffer (paraformaldehyde based)
Fixation
Biolegend Flurofix

Miss. Kara Shumansky

Verified customer

Submitted Mar 04 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, proximal tubule)
Permeabilization
Yes - 1% triton
Specification
kidney, proximal tubule
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

Submitted Feb 25 2019

Answer

Thank you for contacting us.

We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=52460).

For collagen I, we have only one unconjugated antibody that has been tested in flow cytometry. We do, however, have a kit that enables one to easily conjugate the antibody to PE.

Collagen I - ab23446
EasyLink PE - ab102919 or ab102918

For gp100, we have unconjugated antibodies but neither is in a formulation that is compatible with the conjugation kits for FITC. What I recommend is using the unconjugated anti-gp100, ab117853, followed by FITC-conjugated anti-mouse IgG2b, ab98702, pre-adsorbed to remove reactivity with mouse IgG1 (which is the isotype of ab52460, anti-cytokeratin). This should allow you to apply both antibodies to the same sample without cross-reaction of the secondary anti-IgG2b with mouse IgG1.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=117853).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98702).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry and your interest in our products. I can confirm that the ratio is 1:1. I hope this information will be useful for you. Have a nice day,

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