Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, proximal tubule)
Permeabilization
Yes - 1% triton
Specification
kidney, proximal tubule
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 2.5% BSA, .1% triton, PBS
Dilution
1/100

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
The 1:100 dilution used was WAY too strong. I went with this dilution based on what I used to my flow analysis. The image produced was beautiful, but I had to turn the microscope laser way down. I use this antibody at 1:500- 1:1000 dilution for IFF.

Miss. Kara Shumansky

Verified customer

Submitted Feb 25 2019

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