Overview

  • Product name

    Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)]
    See all pan Cytokeratin primary antibodies
  • Description

    Mouse monoclonal [PAN-CK (Cocktail)] to pan Cytokeratin
  • Host species

    Mouse
  • Specificity

    This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) Cytokeratins, with 67kDa (Cytokeratin1); 64kDa (Cytokeratin3); 59kDa (Cytokeratin4); 58kDa (Cytokeratin5); 56kDa (Cytokeratin6); 55kDa (Cytokeratin7); 52kDa (Cytokeratin8); 56.5kDa (Cytokeratin10); 53kDa (Cytokeratin13); 50kDa (Cytokeratin14); 50kDa (Cytokeratin15); 48kDa (Cytokeratin16); 46kDa (Cytokeratin17); 45kDa (Cytokeratin18) and 40kDa (Cytokeratin19).

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Monkey
  • Immunogen

    Full length native protein (purified) corresponding to Human pan Cytokeratin. Human epidermal keratins.

  • Positive control

    • Human skin tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab215838 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    PAN-CK (Cocktail)
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.5-1µg for 106 cells.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 51 kDa.

Target

  • Relevance

    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization

    Cytoplasmic
  • Database links

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling pan Cytokeratin with ab215838 at 1 μg/ml dilution.

References

This product has been referenced in:

  • Chen X  et al. Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages. Biosci Rep 39:N/A (2019). Read more (PubMed: 30760630) »
  • Li Y  et al. Blockage of TGF-a Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells. Biomed Res Int 2019:8231267 (2019). Read more (PubMed: 30906781) »
See all 5 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215838.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up