Overview

  • Product name

    Anti-pan Cytokeratin antibody [PCK-26] (FITC)
    See all pan Cytokeratin primary antibodies
  • Description

    Mouse monoclonal [PCK-26] to pan Cytokeratin (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity


    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Carp, Lizard, Snake, Mammals
  • Immunogen

    Cytokeratin preparation from human epidermis.

Properties

Applications

Our Abpromise guarantee covers the use of ab11214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/25 - 1/50.

Target

  • Relevance

    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • pan ck antibody
    • pan-ck antibody
    • panck antibody

References

This product has been referenced in:

  • Pezzi HM  et al. Integration of Magnetic Bead-Based Cell Selection into Complex Isolations. ACS Omega 3:3908-3917 (2018). Read more (PubMed: 29732449) »
  • Maestre-Batlle D  et al. Novel flow cytometry approach to identify bronchial epithelial cells from healthy human airways. Sci Rep 7:42214 (2017). Read more (PubMed: 28165060) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (HT-29 epitelial,colorectal adenocarcinoma)
Permeabilization
Yes - 0.1% PBS-Triton X-100
Gating Strategy
Gated for all living cells
Specification
HT-29 epitelial,colorectal adenocarcinoma
Fixation
Methanol

Abcam user community

Verified customer

Submitted Feb 08 2019

Answer

Thank you for contacting us.

The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11214 is purified and conjugated to FITC.

The mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted, ab961 is already diluted and ready to use for cell and tissue staining right out of the vial. You receive 6 ml of this pre-diluted antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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