For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM PCK-26 FITC and C-11 FITC staining is weak. Also, I see a lot of nuclear staining. SAMPLE on in vitro grown RPE cells in chamber slides PRIMARY ANTIBODY PCK-26 FITC (1:25-1:50) have tried 30 mins and O/N at 4 degrees C-11 FITC (1:25-1:50) have tried 30 mins and O/N at 4 degrees both made up in 2%BSA/TBS POSITIVE AND NEGATIVE CONTROLS USED No primary antibody ANTIBODY STORAGE CONDITIONS In dark in refrigerator FIXATION OF SAMPLE 1% PFA for ~10 mins BLOCKING CONDITIONS 10% MS in TBS for 20 mins HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 7 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
Asked on Mar 24 2005
Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab11214 and ab11212. What lot numbers did you receive (the lot number is located on the vials)? What was the Abcam order number or purchase order number that was used?
Answered on Mar 28 2005