Key features and details
- Rabbit monoclonal [EPR19409] to Pan-Farnesylation
- Suitable for: Dot blot, WB
- Reacts with: Human
Product nameAnti-Pan-Farnesylation antibody [EPR19409]
See all Pan-Farnesylation primary antibodies
DescriptionRabbit monoclonal [EPR19409] to Pan-Farnesylation
SpecificityThe antibody was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.
Tested applicationsSuitable for: Dot blot, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Pan-Farnesylation aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q15831
- WB: HA-LKB1(WT) transfected 293T lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab199481 in the following tested applications.
|WB||1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
Dot blot analysis of Pan-Farnesylation labeled with ab199481 at 1/1000 dilution.
Lane 1: LKB1 farnesylated peptide;
Lane 2: LKB1 non-farnesylated peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes : Anti-Pan-Farnesylation antibody [EPR19409] (ab199481) at 1/1000 dilution
Lane 1 : HA-LKB1(WT) transfected 293T (Human epithelial cell line from embryonic kidney) lysate
Lane 2 : HA-LKB1(C433S) transfected 293T (Human epithelial cell line from embryonic kidney) lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Exposure time: 2 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by the collaborator (Dr. Sheng-Cai Lin, Xiamen University). Based on the sequence alignment, this product may recognize human, mouse and rat species. The ab was developed specifically for farnesylated LKB1. Refer to the literature (PMID: 24295069), it might also interact with other farnesylated proteins.
ab199481 has not yet been referenced specifically in any publications.